LOCUS       sequence-88916-e        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 548 / 634, 
            Emission: 565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-mOrange-Homer1-N-18
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             491..1201
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1098..1121
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tatcatactt gccaacgtgt aaagtactgt ggaagcacag ctgctgctac agagagcagc
       61 gctgacaagc cgtccgagct ggatggtagt agtcagcaaa tgcggtgcac agcacaagaa
      121 cagagctgag tcagacagtg cagagctgag agaccctaaa gtaaaggaag aggaaataga
      181 aagataaaac aagaaatgat aacgccagag aacttcaaga acagagggac tctttgactc
      241 agaaactaca ggaagttgag attcgaaata aaagacctgg aggggcagct gtcggagctg
      301 gagcagcgcc tggagaagag ccagagcgag caggacgctt ccgcagtaac tgaagactct
      361 cctagagatt ctggacggga aaatatttga actaacagaa ttgcgggata atttggccaa
      421 gctactagaa tgcagcgggt ccagcggttc tagcggctcc gggagtggag gggatccacc
      481 ggtcgccacc atggtgagca agggcgagga gaataatatg gccatcatca aggagttcat
      541 gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga
      601 gggcgagggc cgcccctacg agggctttca gaccgctaag ctgaaggtga ccaagggcgg
      661 ccccctgccc ttcgcctggg acatcctgtc ccctctcttc acctacggct ccaaggccta
      721 cgtgaagcac cccgccgaca tccccgacta cttcaagctg tccttccccg agggcttcaa
      781 gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg accgtgaccc aggactcctc
      841 actgcaggac ggcgagttca tctacaaggt gaagatgcgc ggcaccaact tcccctccga
      901 cggccccgtg atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
      961 cgaggacggc gccctgaagg gcgagatcag gatgaggctg aagctgaagg acggcggcca
     1021 ctacacctcc gaggtcaaga ccacctacaa ggccaagaag tccgtgcagc tgcccggcgc
     1081 ctacatcgtc ggcatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1141 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     1201 agcggccgcg actctagatc ataatcagcc ataccacaat tgtagaagaa atcccc
//
