LOCUS       sequence-88919-e        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 548 / 634, Emission: 565 / 
            662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-mOrange2-MyosinIIB-C-18
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     653..676
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ggcgggctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagaata atatggccat cattaaggag ttcatgcgct tcaaggtgca catggagggc
      121 actgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccaccc ctacgagggc
      181 tttcagaccg ctaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc tcatcaccta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      301 gactacttca agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaactacgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctctctgc aggacggcga gttcatctac
      421 aaggtgaaga tgcgcggcac caacttcccc tccgacggcc ccgtgatgca gaagaagacc
      481 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 atcaggatga ggctgaagct gaaggatggc ggccactaca cctccgaggt caagactacc
      601 tacaaggcca agaagtccgt gctgctgccc ggcgcctaca tcgtcggcat caagctggac
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgctc cgaggcccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggag gtagcgcatc cagtgggggt
      781 gcgtcaggca gtggcagcgg atctggcgcg cagagaactg gactcgagga tccagagagg
      841 tatctctttg tggacagggc tgtcatctac aaccctgcca ctcaagctga ttggacagct
      901 aaaaagctag tgtggattcc atcagaacgc catggttttg aggcagctag tatcaaagag
      961 acggggagat gagttatggt ggagttggca gagatgaaga agcatgtcac aagatgatat
     1021 tcagagatga cccacctagt ttcaggtgag atatgcagat gacatgcttg atgagctcgt
     1081 tactaattct gagatcgctc ctattcagac tatctaactt attctgacct ttcttgtgta
     1141 gttaaagctc agactctcgt tatcctggaa atattgatgt caaggaaggc ctgagttcca
     1201 cctgcattcg aatcgtcaag cgctctcgaa tctgggaaga ctcaat
//
