LOCUS       sequence-88922-e        1298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 548 / 634, Emission: 
            565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1298)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-mOrange2-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..1298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      530..539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             536..1243
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     1143..1166
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tgaaaccaac aaaggcggcc aaggtaatct tccggtcgaa gtggatcctg tcctaagacc
       61 gtctagggat atggataggc ggccggatta cattgttcct ccggaatgga gaggacattc
      121 caggccacac aagttcattg aggctgcagt acaacagcca cctttatgga ccagcgtcca
      181 gttctgtgcg agaggtagca gagcagggaa gcatgcatcc tcgatgttct cgcaatgcgt
      241 gcgcgctgca gcgccacctg caccccatcg ccatctcatc gtcccgctcc tggagatgtg
      301 ctagagatca tagcggatca cagagagcaa gcccggaaag cttcgacaga gcacgaagct
      361 ggagcaggag ttcacagagt gcttctcagc catcgtagag ggcgacagct ttgaagagat
      421 ctatcacaaa gtgaaacgtg tcattgaaga cctctcaggc ccctacatct gggtcccagc
      481 ccgagagaga ctcggcagcg catccggcgg aagcggaagc ggaccggtcg ccaccatggt
      541 gagcaagggc gaggagaata atatggccat cattaaggag ttcatgcgct tcaaggtgca
      601 catggagggc actgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccaccc
      661 ctacgagggc tttcagaccg ctaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      721 ctgggacatc ctgtcccctc tcatcaccta cggctccaag gcctacgtga agcaccccgc
      781 cgacatcccc gactacttca agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      841 gaactacgag gacggcggcg tggtgaccgt gacccaggac tcctctctgc aggacggcga
      901 gttcatctac aaggtgaaga tgcgcggcac caacttcccc tccgacggcc ccgtgatgca
      961 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1021 gaagggcgag atcaggatga ggctgaagct gaaggatggc ggccactaca cctccgaggt
     1081 caagactacc tacaaggcca agaagtccgt gctgctgccc ggcgcctaca tcgtcggcat
     1141 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgctc
     1201 cgaggcccgc cactccaccg gcggcatgga cgagctgtac aagtaagcgg ccgcgactct
     1261 agatcataat cagccatacc acattgtaga aattgcat
//
