LOCUS       sequence-88957-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CDC42-N-10
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(48..620)
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     promoter        complement(655..859)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(684..704)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcgaccggt ggatccccgc ttccacctag cagcacacac
       61 ctgcggctct tcttcggttc tggaggctcc agggcagcca atattgcttc gtcaaataca
      121 ttctttaggc ctttctgtgt aagtgcagaa cactccacat acttgacagc cttcaggtca
      181 cgggccagct tttcagcagt ctctggagtg ataggcttct gtttgttctt ggcaagtttc
      241 tcaatagtag aggggtcatc tctgagatca atttgagtcc caacaagcaa gaaaggagtc
      301 tttggacagt ggtgagttat ctcaggcacc cacttttctt tcacgttttc aaatgaagat
      361 ggagagacca ctgaaaaaca gactagaaat acatctgttt gtggataact cagcggtcgt
      421 aatctgtcat aatcctcttg ccctgcagta tcaaaaagtc caagagtata tggttctcca
      481 ccaatcataa ctgtgactgc atagttgtca aaaacagtcg gtacatattc cgatggaaat
      541 ttgtttgttg tgtaggatat caggagacat gttttaccaa cagcaccatc gcccacaaca
      601 acacacttaa ttgtctgcat ggtggcgcta gcggatctga cggttcacta aaccagctct
      661 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      721 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      781 aatgggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
      841 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
      901 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
      961 tgacgtcaat aggggcgtac ttggcatatg atacacttga tgtactgcca agtgggca
//
