LOCUS       sequence-8896-ex        8083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Groth AC, Fish M, Nusse R, Calos MP
  TITLE     Construction of transgenic Drosophila by using the site-specific 
            integrase from phage phiC31.
  JOURNAL   Genetics. 2004 Apr . 166(4):1775-82.
  PUBMED    15126397
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2004 Apr . 166(4):1775-82."
COMMENT     Sequence Label: pUASTB
FEATURES             Location/Qualifiers
     source          1..8083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(270..288)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(4391..4945,4946..4976)
                     /label=P element 5' end
     rep_origin      complement(5211..5799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5291..5310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6267..6645)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     6592..6611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6830..6934)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7002..7020
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7058..7080)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7180..7199
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(7737..7767,7768..7969)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    join(8049..8083,1..35)
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga
       61 ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac
      121 cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata
      181 cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca agccgaattg
      241 atccactaga aggcctaatt ctagtatgta tgtaagttaa taaaacccat ttttgcggaa
      301 agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat
      361 cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc
      421 tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga
      481 aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt
      541 acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca
      601 agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat
      661 ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa
      721 agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag
      781 caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag
      841 ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc
      901 gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa acagattatt
      961 ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct
     1021 aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta
     1081 agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc
     1141 ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc
     1201 ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg
     1261 caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg
     1321 agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg
     1381 gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc
     1441 aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc
     1501 gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag
     1561 tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa
     1621 agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat
     1681 ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg
     1741 cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccaggaac actgattttc
     1801 caagccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat
     1861 caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc
     1921 agggtgaaag gtctgtccgg cggagaaagg aagcgtcttg ggcatcgctc cgaggctcta
     1981 accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc
     2041 cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc
     2101 attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag
     2161 ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt
     2221 cgatgtgttt attaagggta tctagtatta cataacatct caactcctat ccagcgtggg
     2281 tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt
     2341 gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat
     2401 tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact
     2461 ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt
     2521 cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat
     2581 tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa
     2641 acaaattcgc tagattttag atagaattgc ctgattccac acccttctta gtttttttca
     2701 atgagatgta tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata
     2761 ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc
     2821 tttttgggcc aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc
     2881 ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag
     2941 aatacatttg catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt
     3001 cacctcagag ctgccagttt ttattgaggg aggcccgaag tcgactttat cgctgtgaca
     3061 catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg ccactggtct
     3121 tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact
     3181 gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct
     3241 gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc
     3301 tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     3361 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     3421 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     3481 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     3541 ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac
     3601 gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt accattatta
     3661 ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat
     3721 aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag
     3781 actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaatact
     3841 cgtttatcgc tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg
     3901 cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctctctc
     3961 ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta
     4021 gttccgcgcg attcggttcg ctccaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     4081 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     4141 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     4201 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
     4261 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
     4321 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
     4381 aattaattcg gctgctgctc taaacgacgc atttcgtact ccaaagtacg aattttttcc
     4441 ctcaagctct tattttcatt aaacaatgaa caggacctaa cgcacagtca cgttattgtt
     4501 tacataaatg atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata
     4561 gccttctttt atcttttctg gttcaggctc tatcacttta ctaggtacgg atctgcgttt
     4621 gagtcgcctc cttttaaatg tctgaccttt tgcaggtgca gccttccact gcgaatcatt
     4681 aaagtgggta tcacaaattt gggagttttc accaaggctg cacccaaggc tctgctccca
     4741 caattttctc ttaatagcac acttcggcac gtgaattaat tttactccag tcacagcttt
     4801 gcagcaaaat ttgcaatatt tcattttttt ttattccacg taagggttaa tgttttcaaa
     4861 aaaaaattcg tccgcacaca acctttcctc tcaacaagca aacgtgcact gaatttaagt
     4921 gtatacttcg gtaagcttcg gctatcgacg ggaccacctt atgttatttc atcatgggcc
     4981 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     5041 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     5101 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     5161 aaccatttga ggtatactgg caccgagccc gagttcaaga agaaggcgtt tttccatagg
     5221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5401 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5641 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5881 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5941 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga gcacctatct
     6001 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6061 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6121 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6181 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6241 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6301 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6361 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6421 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6481 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6541 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6601 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6661 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6721 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6781 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6841 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6961 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7021 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     7081 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     7141 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     7201 tgagagtgca ccatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt
     7261 cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga
     7321 aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga
     7381 aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat
     7441 gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg
     7501 ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa
     7561 atataattac attagctttt tgagggggca ataaacagta aacacgatgg taataatggt
     7621 aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg
     7681 aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagaccatg
     7741 atgaaataac ataaggtggt cccgtcggna agagacatcc acttaacgta tgcttgcaat
     7801 aagtgcgagt gaaaggaata gtattctgag tgtcgtattg agtctgagtg agacagcgat
     7861 atgattgttg attaaccctt agcatgtccg tggggtttga attaactcat aatattaatt
     7921 agacgaaatt atttttaaag ttttattttt aataatttgc gagtacgcaa agcttggctg
     7981 catccaacgc gttgggagct ctccggatca attcggcttc aggtaccgtc gacgatgtag
     8041 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc cct
//
