LOCUS       sequence-88963-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith 
            CG, Betzig E
  TITLE     Rapid three-dimensional isotropic imaging of living cells using 
            Bessel beam plane illumination.
  JOURNAL   Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 
            2011 Mar 4.
  PUBMED    21378978
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1586"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "417-23"
COMMENT     Sequence Label: mEmerald-Mito-7
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(55..129)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     promoter        complement(158..361)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(187..207)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        362..665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cccaacgaat ggatcttggc
       61 gcgcggcact gggagccgcc gggccgagcc tgtcaagccc cgcagcagca gcggcgtcag
      121 gacggacatg ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc
      181 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      241 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      301 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      361 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
      421 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
      481 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
      541 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
      601 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
      661 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
      721 aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tca
//
