LOCUS       sequence-88972-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Gao L, Shao L, Higgins CD, Poulton JS, Peifer M, Davidson MW, Wu X, 
            Goldstein B, Betzig E
  TITLE     Noninvasive imaging beyond the diffraction limit of 3D dynamics in 
            thickly fluorescent specimens.
  JOURNAL   Cell. 2012 Dec 7;151(6):1370-85. doi: 10.1016/j.cell.2012.10.008.
  PUBMED    23217717
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.10"; journalName: "Cell"; date: "2012-12-7- 7";
            volume: "151"; issue: "6"; pages: "1370-85"
COMMENT     Sequence Label: mEmerald-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..431
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     polyA_signal    561..682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(598..617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     652..671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(826..847)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcggcatgga cgagctgtac aagtccggac tcagatctgg aggctccgga ggcccagagc
       61 cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga
      121 agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca
      181 aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga
      241 attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt
      301 acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc
      361 ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca
      421 gcgctaagta aggatccacc ggatctagat aactgatcat aatcagccat accacatttg
      481 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      541 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      601 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      661 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
      721 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      781 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      841 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaa
//
