LOCUS       sequence-88975-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    149..270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(186..205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     240..259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(277..732)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(414..435)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     626..645
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        759..863
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(888..908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
       61 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      121 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      241 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
      301 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
      361 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
      421 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
      481 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
      541 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
      601 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
      661 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
      721 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      841 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
      901 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      961 caattagtca gcaaccaggt gtggaaagtc ccc
//
