LOCUS       sequence-8898-ex        8308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Groth AC, Fish M, Nusse R, Calos MP
  TITLE     Construction of transgenic Drosophila by using the site-specific 
            integrase from phage phiC31.
  JOURNAL   Genetics. 2004 Apr . 166(4):1775-82.
  PUBMED    15126397
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2004 Apr . 166(4):1775-82."
COMMENT     Sequence Label: pDrBB2
FEATURES             Location/Qualifiers
     source          1..8308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    29..163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1088..1108
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    1120..1254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1268..1642)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1661..1708)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    1943..2012
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        4052..4331
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             4377..5402
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4377..5402
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5765..5830
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5961..5981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     rep_origin      complement(6456..7044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6536..6555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7215..8006,8007..8075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7838..7857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8076..8180)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctggcgtaat agcgaagagg cccgcaccga tccagacatg ataagataca ttgatgagtt
       61 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      121 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      181 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      241 ctacaaatgt ggtatggctg attatgatcc ccaggaagct cctctgtgtc ctcataaacc
      301 ctaacctcct ctacttgaga ggacattcca atcataggct gcccatccac cctctgtgtc
      361 ctcctgttaa ttaggtcact taacaaaaag gaaattgggt aggggttttt cacagaccgc
      421 tttctaaggg taattttaaa atatctggga agtcccttcc actgctgtgt tccagaagtg
      481 ttggtaaaca gcccacaaat gtcaacagca gaaacataca agctgtcagc tttgcacaag
      541 ggcccaacac cctgctcatc aagaagcact gtggttgctg tgttagtaat gtgcaaaaca
      601 ggaggcacat tttccccacc tgtgtaggtt ccaaaatatc tagtgttttc atttttactt
      661 ggatcaggaa cccagcactc cactggataa gcattatcct tatccaaaac agccttgtgg
      721 tcagtgttca tctgctgact gtcaactgta gcattttttg gggttacagt ttgagcagga
      781 tatttggtcc tgtagtttgc taacacaccc tgcagctcca aaggttcccc accaacagca
      841 aaaaaatgaa aatttgaccc ttgaatgggt tttccagcac cattttcatg agttttttgt
      901 gtccctgaat gcaagtttaa catagcagtt accccaataa cctcagtttt aacagtaaca
      961 gcttcccaca tcaaaatatt tccacaggtt aagtcctcat ttaaattagg caaaggaatt
     1021 cctcgacctg cagcccaagc tcccctttgc atacttctgc ctgctgggga gcctggggac
     1081 tttccacacc ctaactgaca cacattccac agccggatcg atccagacat gataagatac
     1141 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     1201 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agtttcgagg
     1261 tcgagtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
     1321 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
     1381 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
     1441 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
     1501 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
     1561 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
     1621 cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga
     1681 tatactatgc cgatgattaa ttgtcaacac ggtcggggta ccaagcttat cgatggatca
     1741 attcggctgt cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat
     1801 ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt
     1861 tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg
     1921 acccgttcat catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg
     1981 gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc gacggtacct
     2041 gaagccgaat tgatccgagc tcgatgatcc ccctagactt cacagaaagc aagttcctgg
     2101 aatttaatgc agagttggag gaaggaagaa aggaacaaag gtgattgtga tgaccacggc
     2161 tgtaaatatc agcaagcgtg ggaaaacaga ggaaaagtca ggagaaatag acatggctca
     2221 gaatcactgg tacccatcta taatatggaa aagcagatgc tgaactcaaa ttcagggtct
     2281 ggccatcaaa ccacacattc ctcctttttt gttttataaa aatgcgctca gttttatgct
     2341 atatctctgg gagaatgggg aagagccttg ctctgtttat tcaaaaacgt ttctcaaagt
     2401 ggctaggagt tactcctctt agctgcttgg taaggtgttt tacgcataca ggtgataagt
     2461 gatcactttt catgtgacag attattgaag catttatcag ggttattgtc tcatgagcgg
     2521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2581 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2641 gcgtatcacg aggccctttc gtcttcaaga attcacattt gtacgaattt tttttttatc
     2701 aaaagttcga gtttttcacc aatttcctca tcaaccgagc aaggcaaacg gctttgaata
     2761 atatggtgtt atatatacat atatcaaatc gctgctgact gcgtgattga tggccccaag
     2821 attacatatt atcgaatcag gattcagaag gagatcaatg tcaaatgcgg acaggaacat
     2881 gaaagacgcc tgttatgcgc aattaaaaat ttgggtttaa ttgctgtgga aactgttgtt
     2941 ggcggcatct taagttcctg tttaacaaca tcaactactt atgtacgtag aagcgtttaa
     3001 gccatttgca tacagatgag aactggcttt tgtgctaatc agtcaagatg actccgatga
     3061 tgatgactca ttacctgacc agttttcgct gctttctttt caacaactac ttgtatatgt
     3121 attgtatcca atagcaatac attgaatttc catggtctag tcacgtatta tcatttaatt
     3181 gacaccaagt cgtgttattg ttgagctatc gagttcagct caaacatttc ttattcccat
     3241 gaataagccg gcaaaaatat gcaatctatg aaagttaata taagcaaacc ttactttgac
     3301 tcaataccaa tgcactttgt gtcgataggt tcacgcaatt gaggcgatta ttccgataac
     3361 ccaagcgatt gactgttccc gtttcgattc caattgaaat ttggaaatgt acaatagttt
     3421 tgctatatgc tgtcaagtac gctcttatct tctctgggtt ttcttcagag tttcgaaacg
     3481 cttcttcttt tttttgtttt tttttttttg gaatctcgta ttttggaagg ggctcccctc
     3541 tggaatttgt tacactgtcg ttatcattgc gaacaagcgg cccgaagcta tcagcgactt
     3601 taacatttac aatgcacttt tttacgacca attaaatgta cattttcctt tcttcgcccg
     3661 ttgataagcg aacgcgatgt ggcgcaggca atgtgttgct cttgcgacac aaacgcaatc
     3721 aaaatggatt caatttcgct ttttcccagt gaaacgaaga acgaaccgac catcatgata
     3781 tgctcctctg catgttgcgt attgaatcaa tgacaatttc aattaagccg cccgttcgtc
     3841 atgcgttttc gtgcgcttcg aaatgctgat aacggctgct gtcctccaac tgctttgcat
     3901 gtggacacaa ttcccattta tttaattctt ttatttggat cggttaaatt aaaaagcgcc
     3961 ttgttacgca tttaacgttg tttccggtgc gtggtggttt catgcttctg ggaacggcaa
     4021 atgggtttag gattgggaac ccctcatcat ctgttggaat atactattca acctacaaaa
     4081 gtaacgttaa acaacactac tttatatttg atatgaatgg ccacaccttt tatgccataa
     4141 aacatattgt aagagaatac cactcttttt attccttctt tccttcttgt acgttttttg
     4201 ctgtaagtag gtcgtggtgc tggtgttgca gttgaaataa cttaaaatat aaatcataaa
     4261 actcaaacat aaacttgact atttatttat ttattaagaa aggaaatata aattataaat
     4321 tacaacaggt tatggacctg cagccaagct tggcgctcgt ccgggggcaa tgagatatga
     4381 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     4441 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     4501 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     4561 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4621 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4681 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     4741 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     4801 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     4861 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     4921 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     4981 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5041 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5101 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5161 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     5221 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     5281 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     5341 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     5401 agagtagatg ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc
     5461 gagcctagca aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca
     5521 cagttcgcta agctcgctcg gctgggtcgc gggagggccg gtcgcagtga ttcaggccct
     5581 tctggattgt gttggtcccc agggcacgat tgtcatgccc acgcactcgg gtgatctgac
     5641 tgatcccgca gattggagat cgccgcccgt gcctgccgat tgggtgcaga tctttgtgaa
     5701 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     5761 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     5821 tgtattttag attccaacct atggaactga tgaatgggag cagtggtggg aatgccttta
     5881 atgaggaaaa cctgttttgc tcagaagaaa tgccatctan tgatgatgag gctactgctg
     5941 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     6001 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     6061 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     6121 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     6181 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggg ccgacgcgct
     6241 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     6301 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     6361 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcg ccagcaaaag
     6421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7501 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7801 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8221 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     8281 gaattctcat gtttgacagc ttatcatc
//
