LOCUS       sequence-88982-e        3478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych 
            S, Davidson MW, Betzig E
  TITLE     Dual-color superresolution imaging of genetically expressed probes 
            within individual adhesion complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 
            Dec 12.
  PUBMED    18077327
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-12-18"; volume: "104(51)"; pages: 
            "20308-13. Epub 2007 Dec 12"
COMMENT     Sequence Label: mKO-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..3478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2772..2781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2778..3434
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgcaccatca
       61 tggaccatta tgattctcag caaaccaacg attacatgca gccagaagag gactgggacc
      121 gggacctgct cctggacccg gcctgggaga agcagcagag aaagacattc acggcatggt
      181 gtaactccca cctccggaag gcggggacac agatcgagaa catcgaagag gacttccggg
      241 atggcctgaa gctcatgctg ctgctggagg tcatctcagg tgaacgcttg gccaagccag
      301 agcgaggcaa gatgagagtg cacaagatct ccaacgtcaa caaggccctg gatttcatag
      361 ccagcaaagg cgtcaaactg gtgtccatcg gagccgaaga aatcgtggat gggaatgtga
      421 agatgaccct gggcatgatc tggaccatca tcctgcgctt tgccatccag gacatctccg
      481 tggaagagac ttcagccaag gaagggctgc tcctgtggtg tcagagaaag acagcccctt
      541 acaaaaatgt caacatccag aacttccaca taagctggaa ggatggcctc ggcttctgtg
      601 ctttgatcca ccgacaccgg cccgagctga ttgactacgg gaagctgcgg aaggatgatc
      661 cactcacaaa tctgaatacg gcttttgacg tggcagagaa gtacctggac atccccaaga
      721 tgctggatgc cgaagacatc gttggaactg cccgaccgga tgagaaagcc atcatgactt
      781 acgtgtctag cttctaccac gccttctctg gagcccagaa ggcggagaca gcagccaatc
      841 gcatctgcaa ggtgttggcc gtcaaccagg agaacgagca gcttatggaa gactacgaga
      901 agctggccag tgatctgttg gagtggatcc gccgcacaat cccgtggctg gagaaccggg
      961 tgcccgagaa caccatgcat gccatgcaac agaagctgga ggacttccgg gactaccggc
     1021 gcctgcacaa gccgcccaag gtgcaggaga agtgccagct ggagatcaac ttcaacacgc
     1081 tgcagaccaa gctgcggctc agcaaccggc ctgccttcat gccctctgag ggcaggatgg
     1141 tctcggacat caacaatgcc tggggctgcc tggagcaggt ggagaagggc tatgaggagt
     1201 ggttgctgaa tgagatccgg aggctggagc gactggacca cctggcagag aagttccggc
     1261 agaaggcctc catccacgag gcctggactg acggcaaaga ggccatgctg cgacagaagg
     1321 actatgagac cgccaccctc tcggagatca aggccttgct caagaagcat gaggccttcg
     1381 agagtgacct ggctgcccac caggaccgtg tggagcagat tgccgccatc gcacaggagc
     1441 tcaatgagct ggactattat gactcaccca gtgtcaacgc ccgttgccaa aagatctgtg
     1501 accagtggga caatctgggg gccctaactc agaagcgaag ggaagctctg gagcggaccg
     1561 agaaactgct ggagaccatt gaccagctgt acttggagta tgccaagcgg gctgcaccct
     1621 tcaacaactg gatggagggg gccatggagg acctgcagga caccttcatt gtgcacacca
     1681 ttgaggagat ccagggactg accacagccc atgagcagtt caaggccacc ctccctgatg
     1741 ccgacaagga gcgcctggcc atcctgggca tccacaatga ggtgtccaag attgtccaga
     1801 cctaccacgt caatatggcg ggcaccaacc cctacacaac catcacgcct caggagatca
     1861 atggcaaatg ggaccacgtg cggcagctgg tgcctcggag ggaccaagct ctgacggagg
     1921 agcatgcccg acagcagcac aatgagaggc tacgcaagca gtttggagcc caggccaatg
     1981 tcatcgggcc ctggatccag accaagatgg aggagatcgg gaggatctcc attgagatgc
     2041 atgggaccct ggaggaccag ctcagccacc tgcggcagta tgagaagagc atcgtcaact
     2101 acaagccaaa gattgatcag ctggagggcg accaccagct catccaggag gcgctcatct
     2161 tcgacaacaa gcacaccaac tacaccatgg agcacatccg tgtgggctgg gagcagctgc
     2221 tcaccaccat cgccaggacc atcaatgagg tagagaacca gatcctgacc cgggatgcca
     2281 agggcatcag ccaggagcag atgaatgagt tccgggcctc cttcaaccac tttgaccgga
     2341 agaagacagg catgatggac acggatgatt tccgcgcctg cctgatctcc atgggttaca
     2401 acatgggaga agcagaattt gcccgcatca tgagcattgt ggaccccaac cgcctggggg
     2461 tagtgacatt ccaggccttc attgacttca tgtcccgcga gacagccgac acagatacag
     2521 cagaccaagt catggcttcc ttcaagatcc tggctgggga caagaactac attaccatgg
     2581 acgagctgcg ccgcgagctg ccacccgacc aggctgagta ctgcatcgcg cggatggccc
     2641 cctacaccgg ccccgactcc gtgccaggtg ctctggacta catgtccttc tccacggcgc
     2701 tgtacggcga gagtgacctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     2761 atccaccggt cgccaccatg gtgagtgtga ttaaaccaga gatgaagatg aggtactaca
     2821 tggacggctc cgtcaatggg catgagttca caattgaagg tgaaggcaca ggcagacctt
     2881 acgagggaca tcaagagatg acactacgcg tcacaatggc caagggcggg ccaatgcctt
     2941 tcgcgtttga cttagtgtca cacgtgttct gttacggcca cagacctttt actaaatatc
     3001 cagaagagat accagactat ttcaaacaag catttcctga aggcctgtca tgggaaaggt
     3061 cgttggagtt cgaagatggt gggtccgctt cagtcagtgc gcatataagc cttagaggaa
     3121 acaccttcta ccacaaatcc aaatttactg gggttaactt tcctgccgat ggtcctatca
     3181 tgcaaaacca aagtgttgat tgggagccat caaccgagaa aattactgcc agcgacggag
     3241 ttctgaaggg tgatgttacg atgtacctaa aacttgaagg aggcggcaat cacaaatgcc
     3301 aattcaagac tacttacaag gcggcaaaaa agattcttaa aatgccagga agccattaca
     3361 tcagccatcg cctcgtcagg aaaaccgaag gcaacattac tgagctggta gaagatgcag
     3421 tagctcattc ctaagcggcc gcgactctag atcataatca gccataccac atgtagaa
//
