LOCUS       sequence-88988-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..885
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 ggaatcttag tgacgtcaga tccgctagcg ctaccggact cagatctggt accccttgca
       61 gcttccagtt gtcaggaaga gaaggctccc atggatcctg tggtggtgct ggggctctgt
      121 ctctcctgtt tgcttctcct ttcactctgg aaacagagct atgggggagg gaagcttcga
      181 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagtgtg
      241 attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc
      301 acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc
      361 gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc
      421 tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta tttcaaacaa
      481 gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct
      541 tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc caaatttact
      601 ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca
      661 tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta
      721 aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa
      781 aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag gaaaaccgaa
      841 ggcaacatta ctgagctggt agaagatgca gtagctcatt cctaagcggc cgcgactcta
      901 gatcataatc agccatacca cattgtagaa gagcc
//
