LOCUS       sequence-88989-e        1624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 548, Emission: 
            561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Nishimura Y, Applegate K, Davidson MW, Danuser G, Waterman CM
  TITLE     Automated screening of microtubule growth dynamics identifies MARK2 
            as a regulator of leading edge microtubules downstream of Rac1 in 
            migrating cells.
  JOURNAL   PLoS One. 2012;7(7):e41413. doi: 10.1371/journal.pone.0041413. Epub 
            2012 Jul 24.
  PUBMED    22848487
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7):e41413. doi"; pages: " 
            10.1371/journal.pone.0041413. Epub 2012 Jul 24"
COMMENT     Sequence Label: mKO-EB3-7
FEATURES             Location/Qualifiers
     source          1..1624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             929..1585
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc agtcgactgc
       61 caccatggcc gtcaatgtgt actccacatc tgtgaccagt gaaaatctga gtcgccatga
      121 tatgcttgca tgggtcaacg actccctgca cctcaactat accaagatag aacagctttg
      181 ttcaggggca gcctactgcc agttcatgga catgctcttc cccggctgtg tgcacttgag
      241 gaaagtgaag ttccaggcca aactagagca tgaatacatc cacaacttca aggtgctgca
      301 agcagctttc aagaagatgg gtgttgacaa aatcattcct gtagagaaat tagtgaaagg
      361 aaaattccaa gataattttg agtttattca gtggtttaag aaattctttg acgcaaacta
      421 tgatggaaag gattacaacc ctctgctggc gcggcagggc caggacgtag cgccacctcc
      481 taacccaggt gatcagatct tcaacaaatc caagaaactc attggcacag cagttccaca
      541 gaggacgtcc cccacaggcc caaaaaacat gcagacctct ggccggctga gcaatgtggc
      601 ccccccctgc attctccgga agaatcctcc atcagcccga aatggcggcc atgagactga
      661 tgcccaaatt cttgaactca accaacagct ggtggacttg aagctgacag tggatgggct
      721 ggagaaggaa cgtgacttct acttcagcaa acttcgtgac atcgagctca tctgccagga
      781 gcatgaaagt gaaaacagcc ctgttatctc aggcatcatt ggcatcctct atgccacaga
      841 ggaaggattc gcaccccctg aggacgatga gattgaagag catcaacaag aagaccagga
      901 cgagtacctg gatccaccgg tcgccaccat ggtgagtgtg attaaaccag agatgaagat
      961 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac
     1021 aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg ccaagggcgg
     1081 gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagaccttt
     1141 tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc
     1201 atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag
     1261 ccttagagga aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga
     1321 tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc
     1381 cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa
     1441 tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa aagattctta aaatgccagg
     1501 aagccattac atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt
     1561 agaagatgca gtagctcatt cctaagcggc cgcgactcta gatcataatc agccatacca
     1621 catt
//
