LOCUS       sequence-88992-e        1268 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1268)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..1268
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        100..199
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     150..170
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             240..893
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gatgcggttt ggcagtacat caatgggcgt ggatagcggt tgactcacgg ggatttcaag
       61 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      121 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      181 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtacca
      241 tggtgagtgt gattaaacca gagatgaaga tgaggtacta catggacggc tccgtcaatg
      301 ggcatgagtt cacaattgaa ggtgaaggca caggcagacc ttacgaggga catcaagaga
      361 tgacactacg cgtcacaatg gccaagggcg ggccaatgcc tttcgcgttt gacttagtgt
      421 cacacgtgtt ctgttacggc cacagacctt ttactaaata tccagaagag ataccagact
      481 atttcaaaca agcatttcct gaaggcctgt catgggaaag gtcgttggag ttcgaagatg
      541 gtgggtccgc ttcagtcagt gcgcatataa gccttagagg aaacaccttc taccacaaat
      601 ccaaatttac tggggttaac tttcctgccg atggtcctat catgcaaaac caaagtgttg
      661 attgggagcc atcaaccgag aaaattactg ccagcgacgg agttctgaag ggtgatgtta
      721 cgatgtacct aaaacttgaa ggaggcggca atcacaaatg ccaattcaag actacttaca
      781 aggcggcaaa aaagattctt aaaatgccag gaagccatta catcagccat cgcctcgtca
      841 ggaaaaccga aggcaacatt actgagctgg tagaagatgc agtagctcat tcctccggac
      901 tcagatctaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
      961 tgctctcctg agaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga
     1021 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     1081 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     1141 attgcagctt ataatggtta caataaagca atagcatcac aatttcacaa ataagcattt
     1201 tttttcactg cattctagtt gtggtttgtc caactcatca tgtatcttac gcgtaaattg
     1261 tagcgtta
//
