LOCUS       sequence-88993-e        1718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mKO-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..684
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     CDS             complement(1480..1491)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtacc atggtgagtg tgattaaacc agagatgaag
       61 atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga aggtgaaggc
      121 acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat ggccaagggc
      181 gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg ccacagacct
      241 tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc tgaaggcctg
      301 tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag tgcgcatata
      361 agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa ctttcctgcc
      421 gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga gaaaattact
      481 gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga aggaggcggc
      541 aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct taaaatgcca
      601 ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat tactgagctg
      661 gtagaagatg cagtagctca ttcctccgga ctcagatctc gagccatgaa gccaggattc
      721 agtccccgtg ggggtggctt tggcggccga gggggctttg gtgaccgtgg tggtcgtgga
      781 ggccgagggg gctttggcgg gggccgaggc caaggcggag gctttagagg tcgtggacga
      841 ggaggaggtg gaggcggcgg cggcggtgga ggaggaggaa gaggtggtgg aggcttccat
      901 tttggtggca accggggtcg tggtcgggga ggaaaaagag gaaaccagtc ggggaagaat
      961 gtgatggtgg agccgcatcg gcatgagggt gtcttcattt gtcggggaaa ggaagatgca
     1021 ctggtcacca agaacctggt ccctggggaa tcagtttatg gagagaagag agtctcgatt
     1081 tcggaaggag atgacaaaat tgagtaccga gcctggaacc ccttccgctc caagctagca
     1141 gcagcaatcc tgggtggtgt ggaccagatc cacatcaaac cgggggctaa ggttctctac
     1201 ctcggggctg cctcgggcac cacggtctcc catgtctctg acatcgttgg tccggatggt
     1261 ctagtctatg cagtcgagtt ctcccaccgc tctggccgtg acctcattaa cttggccaag
     1321 aagaggacca acatcattcc tgtgatcgag gatgctcgac acccacacaa ataccgcatg
     1381 ctcatcgcaa tggtggatgt gatctttgct gatgtggccc agccagacca gacccggatt
     1441 gtggccctga atgcccacac cttcctgcgt aatggaggac actttgtgat ttccattaag
     1501 gccaactgca ttgactccac agcctcagcc gaggccgtgt ttgcctccga agtgaaaaag
     1561 atgcaacagg agaacatgaa gccgcaggag cagttgaccc ttgagccata tgaaagagac
     1621 catgccgtgg tcgtgggagt gtacaggcca ccccccaagg tgaagaactg aggatccacc
     1681 ggatctagat aactgatcat aatcagccat accacatt
//
