LOCUS       sequence-88994-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Shaner NC, Patterson GH, Davidson MW
  TITLE     Advances in fluorescent protein technology
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
  PUBMED    18057027
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4247-6"
COMMENT     Sequence Label: mKO-Golgi-7
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             283..939
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gtcagatccg ctagcatgag gcttcgggag ccgctcctga gcggcagcgc cgcgatgcca
       61 ggcgcgtccc tacagcgggc ctgccgcctg ctcgtggccg tctgcgctct gcaccttggc
      121 gtcaccctcg tttactacct ggctggccgc gacctgagcc gcctgcccca actggtcgga
      181 gtctccacac cgctgcaggg cggctcgaac agtgccgccg ccatcgggca gtcctccggg
      241 gagctccgga ccggaggggc caaggatcca ccggtcgcca ccatggtgag tgtgattaaa
      301 ccagagatga agatgaggta ctacatggac ggctccgtca atgggcatga gttcacaatt
      361 gaaggtgaag gcacaggcag accttacgag ggacatcaag agatgacact acgcgtcaca
      421 atggccaagg gcgggccaat gcctttcgcg tttgacttag tgtcacacgt gttctgttac
      481 ggccacagac cttttactaa atatccagaa gagataccag actatttcaa acaagcattt
      541 cctgaaggcc tgtcatggga aaggtcgttg gagttcgaag atggtgggtc cgcttcagtc
      601 agtgcgcata taagccttag aggaaacacc ttctaccaca aatccaaatt tactggggtt
      661 aactttcctg ccgatggtcc tatcatgcaa aaccaaagtg ttgattggga gccatcaacc
      721 gagaaaatta ctgccagcga cggagttctg aagggtgatg ttacgatgta cctaaaactt
      781 gaaggaggcg gcaatcacaa atgccaattc aagactactt acaaggcggc aaaaaagatt
      841 cttaaaatgc caggaagcca ttacatcagc catcgcctcg tcaggaaaac cgaaggcaac
      901 attactgagc tggtagaaga tgcagtagct cattcctaag cggccgcgac tctagatcat
      961 aatcagccat accacat
//
