LOCUS       sequence-88997-e        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..684
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtacc atggtgagtg tgattaaacc agagatgaag
       61 atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga aggtgaaggc
      121 acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat ggccaagggc
      181 gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg ccacagacct
      241 tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc tgaaggcctg
      301 tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag tgcgcatata
      361 agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa ctttcctgcc
      421 gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga gaaaattact
      481 gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga aggaggcggc
      541 aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct taaaatgcca
      601 ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat tactgagctg
      661 gtagaagatg cagtagctca ttcctccgga ctcagatctc gagctcaagc ttcgatggcg
      721 actgcgaccc ccgtgccgcc gcggatgggc agccgcgctg gcggccccac cacgccgctg
      781 agccccacgc gcctgtcgcg gctccaggag aaggaggagc tgcgcgagct caatgaccgg
      841 ctggcggtgt acatcgacaa ggtgcgcagc ctggagacgg agaacagcgc gctgcagctg
      901 caggtgacgg agcgcgagga ggtgcgcggc cgtgagctca ccggcctcaa ggcgctctac
      961 gagaccgagc tggccgacgc gcgacgcgcg ctcgacgaca cggcccgcga gcgcgccaag
     1021 ctgcagatcg agctgggcaa gtgcaaggcg gaacacgacc agctgctcct caactatgct
     1081 aagaaggaat ctgatcttaa tggcgcccag atcaagcttc gagaatatga agcagcactc
     1141 aattcgaaag atgcagctct tgctactgca cttggtgaca aaaaaagttt agagggagat
     1201 ttggaggatc tgaaggatca gattgcccag ttggaagcct ccttagctgc agccaaaaaa
     1261 cagttagcag atgaaacttt acttaaagta gatttggaga atcgttgtca gagccttact
     1321 gaggacttgg agtttcgcaa aagcatgtat gaagaggaga ttaacgagac cagaaggaag
     1381 catgaaacgc gcttggtaga ggtggattct gggcgtcaaa ttgagtatga gtacaagctg
     1441 gcgcaagccc ttcatgagat gagagagcaa catgatgccc aagtgaggct gtataaggag
     1501 gagctggagc agacttacca tgccaaactt gagaatgcca gactgtcatc agagatgaat
     1561 acttctactg tcaacagtgc cagggaagaa ctgatggaaa gccgcatgag aattgagagc
     1621 ctttcatccc agctttctaa tctacagaaa gagtctagag catgtttgga aaggattcaa
     1681 gaattagagg acttgcttgc taaagaaaaa gacaactctc gtcgcatgct gacagacaaa
     1741 gagagagaga tggcggaaat aagggatcaa atgcagcaac agctgaatga ctatgaacag
     1801 cttcttgatg taaagttagc cctggacatg gaaatcagtg cttacaggaa actcttagaa
     1861 ggcgaagaag agaggttgaa gctgtctcca agcccttctt cccgtgtgac agtatcccga
     1921 gcatcctcaa gtcgtagtgt acgtacaact agaggaaagc ggaagagggt tgatgtggaa
     1981 gaatcagagg cgagtagtag tgttagcatc tctcattccg cctcagccac tggaaatgtt
     2041 tgcatcgaag aaattgatgt tgatgggaaa tttatccgct tgaagaacac ttctgaacag
     2101 gatcaaccaa tgggaggctg ggagatgatc agaaaaattg gagacacatc agtcagttat
     2161 aaatatacct caagatatgt gctgaaggca ggccagactg ttacaatttg ggctgcaaac
     2221 gctggtgtca cagccagccc cccaactgac ctcatctgga agaaccagaa ctcgtggggc
     2281 actggcgaag atgtgaaggt tatattgaaa aattctcagg gagaggaggt tgctcaaaga
     2341 agtacagtct ttaaaacaac catacctgaa gaagaggagg aggaggaaga agcagctgga
     2401 gtggttgttg aggaagaact tttccaccag cagggaaccc caagagcatc caatagaagc
     2461 tgtgcaatta tgtagggatc caccg
//
