LOCUS       sequence-89-expo        3786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_02
FEATURES             Location/Qualifiers
     source          1..3786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..35
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19..35
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     54..74
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        56..74
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(133..161)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     909..929
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(979..995)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(979..995)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1027..1046)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1028..1046)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1056..1073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1056..1072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1065..1087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1214..1669
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1301..1320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1511..1532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1695..1799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1800..1868,1869..2660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2018..2037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2831..3419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3320..3339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3573..3590
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3707..3728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3743..3748,3749..3766,3767..3773)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(3781..3786,1..11)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(3786,1..22)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
       61 accctcacta aagggaacaa aagctgggta ccggtagaaa aaatgagtaa aggagaagaa
      121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      241 atttgcacta ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact
      301 aaccctgatt atttaaattt tcagccaaca cttgtcacta ctttctgtta tggtgttcaa
      361 tgcttctcga gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc
      421 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      481 aagtttaaac agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc
      541 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      601 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      661 atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact
      721 aactaatctg atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc
      781 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      841 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      901 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      961 ctatacaaat gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
     1021 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     1081 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     1141 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     1201 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1261 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1321 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1381 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1441 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1501 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1561 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     1621 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     1681 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1741 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1801 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1861 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1921 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1981 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2041 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2101 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2161 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2221 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2281 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2341 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2401 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2461 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     2521 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2581 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2641 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2701 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2761 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2821 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2881 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2941 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3001 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3061 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3121 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3181 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3241 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3301 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3361 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3421 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3481 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3541 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3601 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3661 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     3721 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     3781 ttgtga
//
