LOCUS       sequence-89001-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Contacts, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych 
            S, Davidson MW, Betzig E
  TITLE     Dual-color superresolution imaging of genetically expressed probes 
            within individual adhesion complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 
            Dec 12.
  PUBMED    18077327
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-12-18"; volume: "104(51)"; pages: 
            "20308-13. Epub 2007 Dec 12"
COMMENT     Sequence Label: mKO-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..241
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             255..911
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc tgcatcactg
       61 ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc aagcagctca
      121 acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc tttctcaaac
      181 tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      241 caccggtcgc caccatggtg agtgtgatta aaccagagat gaagatgagg tactacatgg
      301 acggctccgt caatgggcat gagttcacaa ttgaaggtga aggcacaggc agaccttacg
      361 agggacatca agagatgaca ctacgcgtca caatggccaa gggcgggcca atgcctttcg
      421 cgtttgactt agtgtcacac gtgttctgtt acggccacag accttttact aaatatccag
      481 aagagatacc agactatttc aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt
      541 tggagttcga agatggtggg tccgcttcag tcagtgcgca tataagcctt agaggaaaca
      601 ccttctacca caaatccaaa tttactgggg ttaactttcc tgccgatggt cctatcatgc
      661 aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat tactgccagc gacggagttc
      721 tgaagggtga tgttacgatg tacctaaaac ttgaaggagg cggcaatcac aaatgccaat
      781 tcaagactac ttacaaggcg gcaaaaaaga ttcttaaaat gccaggaagc cattacatca
      841 gccatcgcct cgtcaggaaa accgaaggca acattactga gctggtagaa gatgcagtag
      901 ctcattccta agcggccgcg actctagatc ataatcagcc ataccacatt gaga
//
