LOCUS       sequence-89003-e        2174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 548, Emission: 
            561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2174)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1479..2135
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccca
       61 tgtccaccag gtccgtgtcc tcgtcctcct accgcaggat gttcggcggc ccgggcaccg
      121 cgagccggcc gagctccagc cggagctacg tgactacgtc cacccgcacc tacagcctgg
      181 gcagcgcgct gcgccccagc accagccgca gcctctacgc ctcgtccccg ggcggcgtgt
      241 atgccacgcg ctcctctgcc gtgcgcctgc ggagcagcgt gcccggggtg cggctcctgc
      301 aggactcggt ggacttctcg ctggccgacg ccatcaacac cgagttcaag aacacccgca
      361 ccaacgagaa ggtggagctg caggagctga atgaccgctt cgccaactac atcgacaagg
      421 tgcgcttcct ggagcagcag aataagatcc tgctggccga gctcgagcag ctcaagggcc
      481 aaggcaagtc gcgcctgggg gacctctacg aggaggagat gcgggagctg cgccggcagg
      541 tggaccagct aaccaacgac aaagcccgcg tcgaggtgga gcgcgacaac ctggccgagg
      601 acatcatgcg cctccgggag aaattgcagg aggagatgct tcagagagag gaagccgaaa
      661 acaccctgca atctttcaga caggatgttg acaatgcgtc tctggcacgt cttgaccttg
      721 aacgcaaagt ggaatctttg caagaagaga ttgccttttt gaagaaactc cacgaagagg
      781 aaatccagga gctgcaggct cagattcagg aacagcatgt ccaaatcgat gtggatgttt
      841 ccaagcctga cctcacggct gccctgcgtg acgtacgtca gcaatatgaa agtgtggctg
      901 ccaagaacct gcaggaggca gaagaatggt acaaatccaa gtttgctgac ctctctgagg
      961 ctgccaaccg gaacaatgac gccctgcgcc aggcaaagca ggagtccact gagtaccgga
     1021 gacaggtgca gtccctcacc tgtgaagtgg atgcccttaa aggaaccaat gagtccctgg
     1081 aacgccagat gcgtgaaatg gaagagaact ttgccgttga agctgctaac taccaagaca
     1141 ctattggccg cctgcaggat gagattcaga atatgaagga ggaaatggct cgtcaccttc
     1201 gtgaatacca agacctgctc aatgttaaga tggcccttga cattgagatt gccacctaca
     1261 ggaagctgct ggaaggcgag gagagcagga tttctctgcc tcttccaaac ttttcctccc
     1321 tgaacctgag ggaaactaat ctggattcac tccctctggt tgatacccac tcaaaaagga
     1381 cacttctgat taagacggtt gaaactagag atggacaggt tatcaacgaa acttctcagc
     1441 atcacgatga ccttgaaggg gatccaccgg tcgccaccat ggtgagtgtg attaaaccag
     1501 agatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag
     1561 gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg
     1621 ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc
     1681 acagaccttt tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg
     1741 aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg
     1801 cgcatataag ccttagagga aacaccttct accacaaatc caaatttact ggggttaact
     1861 ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga
     1921 aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag
     1981 gaggcggcaa tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa aagattctta
     2041 aaatgccagg aagccattac atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta
     2101 ctgagctggt agaagatgca gtagctcatt cctaagcggc cgcgactcta gatcataatc
     2161 agccatacca catt
//
