LOCUS       sequence-89004-e        3981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: mKO-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..682
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 ggacgtcaga tccgctagca ttgccaccat ggtgagtgtg attaaaccag agatgaagat
       61 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac
      121 aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg ccaagggcgg
      181 gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagaccttt
      241 tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc
      301 atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag
      361 ccttagagga aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga
      421 tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc
      481 cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa
      541 tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa aagattctta aaatgccagg
      601 aagccattac atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt
      661 agaagatgca gtagctcatt ccggatcctc aggtaccgga actgcagcag agaattcggg
      721 aaactcgaga acaaagcttg aaaacggccg catgccagtg tttcatacgc gcacgatcga
      781 gagcatcctg gagccggtgg cacagcagat ctcccacctg gtgataatgc acgaggaggg
      841 cgaggtggac ggcaaagcca ttcctgacct caccgcgccc gtggccgccg tgcaggcggc
      901 cgtcagcaac ctcgtccggg ttggaaaaga gactgttcaa accactgagg atcagatttt
      961 gaagagagat atgccaccag catttattaa ggttgagaat gcttgcacca agcttgtcca
     1021 ggcagctcag atgcttcagt cagaccctta ctcagtgcct gctcgagatt atctaattga
     1081 tgggtcaagg ggcatcctct ctggaacatc agacctgctc cttaccttcg atgaggctga
     1141 ggtccgtaaa attattagag tttgcaaagg aattttggaa tatcttacag tggcagaggt
     1201 ggtggagact atggaagatt tggtcactta cacaaagaat cttgggccag gaatgactaa
     1261 gatggccaag atgattgacg agagacagca ggagctcact caccaggagc accgagtgat
     1321 gttggtgaac tcgatgaaca ccgtgaaaga gttgctgcca gttctcattt cagctatgaa
     1381 gatttttgta acaactaaaa actcaaaaaa ccaaggcata gaggaagctt taaaaaatcg
     1441 caattttact gtagaaaaaa tgagtgctga aattaatgag ataattcgtg tgttacaact
     1501 cacctcttgg gatgaagatg cctgggccag caaggacact gaagccatga agagagcatt
     1561 ggcctccata gactccaaac tgaaccaggc caaaggttgg ctccgtgacc ctagtgcctc
     1621 cccaggggat gctggtgagc aggccatcag acagatctta gatgaagctg gaaaagttgg
     1681 tgaactctgt gcaggcaaag aacgcaggga gattctggga acttgcaaaa tgctagggca
     1741 gatgactgat caagtggctg acctccgtgc cagaggacaa ggatcctcac cggtggccat
     1801 gcagaaagct cagcaggtat ctcagggtct ggatgtgctc acagcaaaag tggaaaatgc
     1861 agctcgcaag ctggaagcca tgaccaactc aaagcagagc attgcaaaga agatcgatgc
     1921 tgctcagaac tggcttgcag atccaaatgg tggaccggaa ggagaagagc agattcgagg
     1981 tgctttggct gaagctcgga aaatagcaga attatgtgat gatcctaaag aaagagatga
     2041 cattctacgt tcccttgggg aaatatctgc tctgacttct aaattagcag atctacgaag
     2101 acaggggaaa ggagattctc cagaggctcg agccttggcc aaacaggtgg ccacggccct
     2161 gcagaacctg cagaccaaaa ccaaccgggc tgtggccaac agcagaccgg ccaaagcagc
     2221 tgtacacctt gagggcaaga ttgagcaagc acagcggtgg attgataatc ccacagtgga
     2281 tgaccgtgga gtcggtcagg ctgccatccg ggggcttgtg gccgaagggc atcgtctggc
     2341 taatgttatg atggggcctt atcggcaaga tcttctcgcc aagtgtgacc gagtggacca
     2401 gctgacagcc cagctggctg acctggctgc cagaggggaa ggggagagtc ctcaggcacg
     2461 agcacttgca tctcagctcc aagactcctt aaaggatcta aaagctcgga tgcaggaggc
     2521 catgactcag gaagtgtcag atgttttcag cgataccaca actcccatca agctgttggc
     2581 agtggcagcc acggcgcctc ctgatgcgcc taacagggaa gaggtatttg atgagagggc
     2641 agctaacttt gaaaaccatt caggaaagct tggtgctacg gccgagaagg cggctgcggt
     2701 tggtactgct aataaatcaa cagtggaagg cattcaggcc tcagtgaaga cggcccgaga
     2761 actcacaccc caggtggtct cggctgctcg tatcttactt aggaaccctg gaaatcaagc
     2821 tgcttatgaa cattttgaga ccatgaagaa ccagtggatc gataatgttg aaaaaatgac
     2881 agggctggtg gacgaagcca ttgataccaa atctctgttg gatgcttcag aagaagcaat
     2941 taaaaaagac ctggacaagt gcaaggtagc tatggccaac attcagcctc agatgctggt
     3001 tgctggggca accagtattg ctcgtcgggc caaccggatc ctgctggtgg ctaagaggga
     3061 ggtggagaat tccgaggatc ccaagttccg tgaggctgtg aaagctgcct ctgatgaatt
     3121 gagcaaaacc atctccccga tggtgatgga tgcaaaagct gtggctggaa acatttccga
     3181 ccctggactg caaaagagct tcctggactc aggatatcgg atcctgggag ctgtggccaa
     3241 ggtcagagaa gccttccaac ctcaggagcc tgacttcccg ccgcctccac cagaccttga
     3301 acaactccga ctaacagatg agcttgctcc tcccaaacca cctctgcctg aaggtgaggt
     3361 ccctccacct aggcctccac caccagagga aaaggatgaa gagttccctg agcagaaggc
     3421 cggggaggtg attaaccagc caatgatgat ggctgccaga cagctccatg atgaagctcg
     3481 caaatggtcc agcaagggca atgacatcat tgcagcagcc aagcgcatgg ctctgctgat
     3541 ggctgagatg tctcggctgg taagaggggg cagtggtacc aagcgggcac tcattcagtg
     3601 tgccaaggac atcgccaagg cctcagatga ggtgactcgg ttggccaagg aggttgccaa
     3661 gcagtgcaca gataaacgga ttagaaccaa cctcttacag gtatgtgagc gaatcccaac
     3721 cataagcacc cagctcaaaa tcctgtccac agtgaaggcc accatgctgg gccggaccaa
     3781 catcagtgat gaggagtctg agcaggccac agagatgctg gttcacaatg cccagaacct
     3841 catgcagtct gtgaaggaga ctgtgcggga agctgaagct gcttcaatca aaattcgaac
     3901 agatgctgga tttacactgc gctgggttag aaagactccc tggtaccagt aggcggccgc
     3961 gactctagac ataatcagcc a
//
