LOCUS       sequence-89005-e        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych 
            S, Davidson MW, Betzig E
  TITLE     Dual-color superresolution imaging of genetically expressed probes 
            within individual adhesion complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 
            Dec 12.
  PUBMED    18077327
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-12-18"; volume: "104(51)"; pages: 
            "20308-13. Epub 2007 Dec 12"
COMMENT     Sequence Label: mKO-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1786..1795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1792..2448
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 aacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccatg
       61 gcggcccccc gcccgtctcc cgcgatcccc gtttcggtct cggctccggc tttttacgcc
      121 ccgcagaaga agttcggccc tgtggtggcc ccaaagccca aagtgaatcc cttccggccc
      181 agggacagcg agcctccccc ggcacccggg gcccagcgcg cacagatggg ccgggtgggc
      241 gatattcccc cgccgccccc ggaagacttt cccctgcctc cacctcccct tgctggggat
      301 ggcgacgatg cagagggtgc tctgggaggt gccttcccgc cgccccctcc cccgatcgag
      361 gaatcatttc cccctgcgcc tctggaggag gagatcttcc cttccccgcc gcctcctccg
      421 gaggaggagg gagggcctga ggcccccata ccgcccccac cacagcccag ggagaaggtg
      481 agcagtattg atttggagat cgactctctg tcctcactgc tggatgacat gaccaagaat
      541 gatcctttca aagcccgggt gtcatctgga tatgtgcccc caccagtggc cactccattc
      601 agttccaagt ccagtaccaa gcctgcagcc gggggcacag cacccctgcc tccttggaag
      661 tccccttcca gctcccagcc tctgccccag gttccggctc cggctcagag ccagacacag
      721 ttccatgttc agccccagcc ccagcccaag cctcaggtcc aactccatgt ccagtcccag
      781 acccagcctg tgtctttggc taacacccag ccccgagggc ccccagcctc atctccggct
      841 ccagccccta agttttctcc agtgactcct aagtttactc ctgtggcttc caagttcagt
      901 cctggagccc caggtggatc tgggtcacaa ccaaatcaaa aattggggca ccccgaagct
      961 ctttctgctg gcacaggctc ccctcaacct cccagcttca cctatgccca gcagagggag
     1021 aagccccgag tgcaggagaa gcagcacccc gtgcccccac cggctcagaa ccaaaaccag
     1081 gtgcgctccc ctggggcccc agggcccctg actctgaagg aggtggagga gctggagcag
     1141 ctgacccagc agctaatgca ggacatggag catcctcaga ggcagaatgt ggctgtcaac
     1201 gaactctgcg gccgatgcca tcaacccctg gcccgggcgc agccagccgt ccgcgctcta
     1261 gggcagctgt tccacatcgc ctgcttcacc tgccaccagt gtgcgcagca gctccagggc
     1321 cagcagttct acagtctgga gggggcgccg tactgcgagg gctgttacac tgacaccctg
     1381 gagaagtgta acacctgcgg ggagcccatc actgaccgca tgctgagggc cacgggcaag
     1441 gcctatcacc cgcactgctt cacctgtgtg gtctgcgccc gccccctgga gggcacctcc
     1501 ttcatcgtgg accaggccaa ccggccccac tgtgtccccg actaccacaa gcagtacgcc
     1561 ccgaggtgct ccgtctgctc tgagcccatc atgcctgagc ctggccgaga tgagactgtg
     1621 cgagtggtcg ccctggacaa gaacttccac atgaagtgtt acaagtgtga ggactgcggg
     1681 aagcccctgt cgattgaggc agatgacaat ggctgcttcc ccctggacgg tcacgtgctc
     1741 tgtcggaagt gccacactgc tagagcccag acggatccac cggtcgccac catggtgagt
     1801 gtgattaaac cagagatgaa gatgaggtac tacatggacg gctccgtcaa tgggcatgag
     1861 ttcacaattg aaggtgaagg cacaggcaga ccttacgagg gacatcaaga gatgacacta
     1921 cgcgtcacaa tggccaaggg cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg
     1981 ttctgttacg gccacagacc ttttactaaa tatccagaag agataccaga ctatttcaaa
     2041 caagcatttc ctgaaggcct gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc
     2101 gcttcagtca gtgcgcatat aagccttaga ggaaacacct tctaccacaa atccaaattt
     2161 actggggtta actttcctgc cgatggtcct atcatgcaaa accaaagtgt tgattgggag
     2221 ccatcaaccg agaaaattac tgccagcgac ggagttctga agggtgatgt tacgatgtac
     2281 ctaaaacttg aaggaggcgg caatcacaaa tgccaattca agactactta caaggcggca
     2341 aaaaagattc ttaaaatgcc aggaagccat tacatcagcc atcgcctcgt caggaaaacc
     2401 gaaggcaaca ttactgagct ggtagaagat gcagtagctc attcctaagc ggccgcgact
     2461 ctagatcata atcagccata ccaca
//
