LOCUS       sequence-89006-e        1882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1882)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Actin-7
FEATURES             Location/Qualifiers
     source          1..1882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg
       61 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      121 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      181 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      241 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      301 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      361 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      421 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      481 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      541 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      601 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      661 caggtagaag atgcagtagc tcatagctcc ggactcagat ctcgagctat ggatgatgat
      721 atcgccgcgc tcgtcgtcga caacggctcc ggcatgtgca aggccggctt cgcgggcgac
      781 gatgcccccc gggccgtctt cccctccatc gtggggcgcc ccaggcacca gggcgtgatg
      841 gtgggcatgg gtcagaagga ttcctatgtg ggcgacgagg cccagagcaa gagaggcatc
      901 ctcaccctga agtaccccat cgagcacggc atcgtcacca actgggacga catggagaaa
      961 atctggcacc acaccttcta caatgagctg cgtgtggctc ccgaggagca ccccgtgctg
     1021 ctgaccgagg cccccctgaa ccccaaggcc aaccgcgaga agatgaccca gatcatgttt
     1081 gagaccttca acaccccagc catgtacgtt gctatccagg ctgtgctatc cctgtacgcc
     1141 tctggccgta ccactggcat cgtgatggac tccggtgacg gggtcaccca cactgtgccc
     1201 atctacgagg ggtatgccct cccccatgcc atcctgcgtc tggacctggc tggccgggac
     1261 ctgactgact acctcatgaa gatcctcacc gagcgcggct acagcttcac caccacggcc
     1321 gagcgggaaa tcgtgcgtga cattaaggag aagctgtgct acgtcgccct ggacttcgag
     1381 caagagatgg ccacggctgc ttccagctcc tccctggaga agagctacga gctgcctgac
     1441 ggccaggtca tcaccattgg caatgagcgg ttccgctgcc ctgaggcact cttccagcct
     1501 tccttcctgg gcatggagtc ctgtggcatc cacgaaacta ccttcaactc catcatgaag
     1561 tgtgacgtgg acatccgcaa agacctgtac gccaacacag tgctgtctgg cggcaccacc
     1621 atgtaccctg gcattgccga caggatgcag aaggagatca ctgccctggc acccagcaca
     1681 atgaagatca agatcattgc tcctcctgag cgcaagtact ccgtgtggat cggcggctcc
     1741 atcctggcct cgctgtccac cttccagcag atgtggatca gcaagcagga gtatgacgag
     1801 tccggcccct ccatcgtcca ccgcaaatgc ttctagggat ccaccggatc tagataactg
     1861 atcataatca gccataccac at
//
