LOCUS       sequence-89007-e        1914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1914)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg
       61 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      121 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      181 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      241 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      301 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      361 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      421 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      481 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      541 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      601 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      661 caggtagaag atgcagtagc tcatagctcc ggactcagat ctggcagcgg tggaggcagc
      721 gcatccggcg gaagcggaag cgatgatgat atcgccgcgc tcgtcgtcga caacggctcc
      781 ggcatgtgca aggccggctt cgcgggcgac gatgcccccc gggccgtctt cccctccatc
      841 gtggggcgcc ccaggcacca gggcgtgatg gtgggcatgg gtcagaagga ttcctatgtg
      901 ggcgacgagg cccagagcaa gagaggcatc ctcaccctga agtaccccat cgagcacggc
      961 atcgtcacca actgggacga catggagaaa atctggcacc acaccttcta caatgagctg
     1021 cgtgtggctc ccgaggagca ccccgtgctg ctgaccgagg cccccctgaa ccccaaggcc
     1081 aaccgcgaga agatgaccca gatcatgttt gagaccttca acaccccagc catgtacgtt
     1141 gctatccagg ctgtgctatc cctgtacgcc tctggccgta ccactggcat cgtgatggac
     1201 tccggtgacg gggtcaccca cactgtgccc atctacgagg ggtatgccct cccccatgcc
     1261 atcctgcgtc tggacctggc tggccgggac ctgactgact acctcatgaa gatcctcacc
     1321 gagcgcggct acagcttcac caccacggcc gagcgggaaa tcgtgcgtga cattaaggag
     1381 aagctgtgct acgtcgccct ggacttcgag caagagatgg ccacggctgc ttccagctcc
     1441 tccctggaga agagctacga gctgcctgac ggccaggtca tcaccattgg caatgagcgg
     1501 ttccgctgcc ctgaggcact cttccagcct tccttcctgg gcatggagtc ctgtggcatc
     1561 cacgaaacta ccttcaactc catcatgaag tgtgacgtgg acatccgcaa agacctgtac
     1621 gccaacacag tgctgtctgg cggcaccacc atgtaccctg gcattgccga caggatgcag
     1681 aaggagatca ctgccctggc acccagcaca atgaagatca agatcattgc tcctcctgag
     1741 cgcaagtact ccgtgtggat cggcggctcc atcctggcct cgctgtccac cttccagcag
     1801 atgtggatca gcaagcagga gtatgacgag tccggcccct ccatcgtcca ccgcaaatgc
     1861 ttctagggat ccaccggatc tagataactg atcataatca gccataccac attg
//
