LOCUS       sequence-89010-e        2540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2540)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1846..1855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1852..2508
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctagcgctac cggactcaga tctcgagacc gccatggaag ggaagtggtt gctgtgtatg
       61 ttactggtgc ttggaactgc tattgttgag gctcatgatg gacatgatga tgatgtgatt
      121 gatattgagg atgaccttga cgatgtcatt gaagaggtag aagactcaaa accagatacc
      181 actgctcctc cttcatctcc caaggttact tacaaagctc cagttccaac aggggaagta
      241 tattttgctg attcttttga cagaggaact ctgtcagggt ggattttatc caaagccaag
      301 aaagacgata ccgatgatga aattgccaaa tatgatggaa agtgggaggt agaggaaatg
      361 aaggagtcaa agcttccagg tgataaagga cttgtgttga tgtctcgggc caagcatcat
      421 gccatctctg ctaaactgaa caagcccttc ctgtttgaca ccaagcctct cattgttcag
      481 tatgaggtta atttccaaaa tggaatagaa tgtggtggtg cctatgtgaa actgctttct
      541 aaaacaccag aactcaacct ggatcagttc catgacaaga ccccttatac gattatgttt
      601 ggtccagata aatgtggaga ggactataaa ctgcacttca tcttccgaca caaaaacccc
      661 aaaacgggta tctatgaaga aaaacatgct aagaggccag atgcagatct gaagacctat
      721 tttactgata agaaaacaca tctttacaca ctaatcttga atccagataa tagttttgaa
      781 atactggttg accaatctgt ggtgaatagt ggaaatctgc tcaatgacat gactcctcct
      841 gtaaatcctt cacgtgaaat tgaggaccca gaagaccgga agcccgagga ttgggatgaa
      901 agaccaaaaa tcccagatcc agaagctgtc aagccagatg actgggatga agatgcccct
      961 gctaagattc cagatgaaga ggccacaaaa cccgaaggct ggttagatga tgagcctgag
     1021 tacgtacctg atccagacgc agagaaacct gaggattggg atgaagacat ggatggagaa
     1081 tgggaggctc ctcagattgc caaccctaga tgtgagtcag ctcctggatg tggtgtctgg
     1141 cagcgacctg tgattgacaa ccccaattat aaaggcaaat ggaagcctcc tatgattgac
     1201 aatcccagtt accagggaat ctggaaaccc aggaaaatac caaatccaga tttctttgaa
     1261 gatctggaac ctttcagaat gactcctttt agtgctattg gtttggagct gtggtccatg
     1321 acctctgaca ttttttttga caactttatc atttgtgctg atcgaagaat agttgatgat
     1381 tgggccaatg atggatgggg cctgaagaaa gctgctgatg gggctgctga gccaggcgtt
     1441 gtggggcaga tgaacgaggc agctgaagag cgcccgtggc tgtgggtagt ctatattcta
     1501 actgtagccc ttcctgtgtt cctggttatc ctcttctgct gttctggaaa gaaacagacc
     1561 agtggtatgg agtataagaa aactgatgca cctcaaccgg atgtgaagga agaggaagaa
     1621 gagaaggaag aggaaaagga caagggagat gaggaggagg aaggagaaga gaaacttgaa
     1681 gagaaacaga aaagtgatgc tgaagaagat ggtggcactg tcagtcaaga ggaggaagac
     1741 agaaaaccta aagcagagga ggatgaaatt ttgaacagat caccaagaaa cagaaagcca
     1801 cgaagagagg ggggtgcgtc aggcggcagc ggatctggac cggtcgccac catggtgagt
     1861 gtgattaaac cagagatgaa gatgaggtac tacatggacg gctccgtcaa tgggcatgag
     1921 ttcacaattg aaggtgaagg cacaggcaga ccttacgagg gacatcaaga gatgacacta
     1981 cgcgtcacaa tggccgaggg cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg
     2041 ttctgttacg gccacagagt atttactaaa tatccagaag agataccaga ctatttcaaa
     2101 caagcatttc ctgaaggcct gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc
     2161 gcttcagtca gtgcgcatat aagccttaga ggaaacacct tctaccacaa atccaaattt
     2221 actggggtta actttcctgc cgatggtcct atcatgcaaa accaaagtgt tgattgggag
     2281 ccatcaaccg agaaaattac tgccagcgac ggagttctga agggtgatgt tacgatgtac
     2341 ctaaaacttg aaggaggcgg caatcacaaa tgccaaatga agactactta caaggcggca
     2401 aaagagattc ttgaaatgcc aggagaccat tacatcggcc atcgcctcgt caggaaaacc
     2461 gaaggcaaca ttactgagca ggtagaagat gcagtagctc atagctaagc ggccgcgact
     2521 ctagatcata atcagccata
//
