LOCUS       sequence-89011-e        1331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1331)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggactcaga tctggcagca gtggaggttc
      721 tgggggcaaa tacgtagact cggagggaca tctctacacc gttcccatcc gggaacaggg
      781 caacatctac aagcccaaca acaaggccat ggcagacgag ctgagcgaga agcaagtgta
      841 cgacgcgcac accaaggaga tcgacctggt caaccgcgac cctaaacacc tcaacgatga
      901 cgtggtcaag attgactttg aagatgtgat tgcagaacca gaagggacac acagttttga
      961 cggcatttgg aaggccagct tcaccacctt cactgtgacg aaatactggt tttaccgctt
     1021 gctgtctgcc ctctttggca tcccgatggc actcatctgg ggcatttact tcgccattct
     1081 ctctttcctg cacatctggg cagttgtacc atgcattaag agcttcctga ttgagattca
     1141 gtgcatcagc cgtgtctatt ccatctacgt ccacaccgtc tgtgacccac tctttgaagc
     1201 tgttgggaaa atattcagca atgtccgcat caacttgcag aaagaaatat aagaattctg
     1261 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     1321 ataccacatt t
//
