LOCUS       sequence-89013-e        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-CEBPA237-C-4
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..684
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagtg tgattaaacc agagatgaag
       61 atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga aggtgaaggc
      121 acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat ggccgagggc
      181 gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg ccacagagta
      241 tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc tgaaggcctg
      301 tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag tgcgcatata
      361 agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa ctttcctgcc
      421 gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga gaaaattact
      481 gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga aggaggcggc
      541 aatcacaaat gccaaatgaa gactacttac aaggcggcaa aagagattct tgaaatgcca
      601 ggagaccatt acatcggcca tcgcctcgtc aggaaaaccg aaggcaacat tactgagcag
      661 gtagaagatg cagtagctca tagctccgga cctgcaatgg gtgctgcggg cctgccgggc
      721 cccgggggct cgctcaaggg cttggctggt ccgcaccccg acctccgcac cggcggcggc
      781 ggcggcggcg gggccggcgc gggcaaggcc aagaagtcgg tggataagaa cagcaacgag
      841 taccgggtac ggcgggaacg caacaacatc gcggtgcgca agagccgaga taaagccaaa
      901 cagcgcaacg tggagacgca gcagaaggtg ttggagttga ccagtgacaa tgaccgcctg
      961 cgcaagcggg tggaacagct gagccgtgaa ctggacacgc tgcggggtat cttccgccag
     1021 ctgcctgaga gctccttggt caaggccatg ggcaactgcg cgtgaggcgc gcggatccac
     1081 cggatctaga taactgatca taatcagcca taccacatt
//
