LOCUS       sequence-89017-e        1499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1499)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             804..1460
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcgg
       61 ctgcacgctc ctcgggacac agtgccaacc atccagagga caagatggat tggggcacac
      121 tacagagcat cctcgggggt gtcaacaagc actccaccag cattgggaaa atctggctca
      181 ctgtcctctt catcttccgc atcatgatcc tcgtggtggc cgcgaaggag gtgtggggag
      241 atgagcaagc cgattttgtt tgcaacactc tccagcctgg ctgtaagaat gtgtgctacg
      301 accactactt ccccatctct cacatccggc tctgggctct gcagctgatc atggtgtcca
      361 cgccggccct cctggtagct atgcacgtgg cctaccggag acacgaaaag aaacggaagt
      421 tcatgaaggg agagataaag aacgagttta aggacatcga agagatcaaa acccagaagg
      481 tccgtatcga agggtccctg tggtggacct acaccaccag catcttcttc cgggtcatct
      541 tcgaagctgt cttcatgtat gtcttttaca tcatgtacaa tggcttcttc atgcagcgtc
      601 tggtgaagtg taacgcctgg ccttgtccca atacagtgga ctgcttcatt tccaggccca
      661 cagaaaagac tgtcttcacg gtgttcatga tctctgtgtc tggaatttgc atcctgctaa
      721 acatcacaga gctgtgctat ctgttcatta ggtattgctc agggaagtcc aaaagaccag
      781 tcgcggatcc accggtcgcc accatggtga gtgtgattaa accagagatg aagatgaggt
      841 actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa ggcacaggca
      901 gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag ggcgggccaa
      961 tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga gtatttacta
     1021 aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc ctgtcatggg
     1081 aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat ataagcctta
     1141 gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct gccgatggtc
     1201 ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt actgccagcg
     1261 acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc ggcaatcaca
     1321 aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg ccaggagacc
     1381 attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag caggtagaag
     1441 atgcagtagc tcatagctaa gcggccgcga ctctagatca taatcagcca taccacatt
//
