LOCUS       sequence-89018-e        1688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1688)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             989..1645
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccctc
       61 tgggaaaggg cagcagcagc caggtgtggc agtgacaggg aggtgtgaat gaggcaggat
      121 gaactggaca ggtttgtaca ccttgctcag tggcgtgaac cggcattcta ctgccattgg
      181 ccgagtatgg ctctcggtca tcttcatctt cagaatcatg gtgctggtgg tggctgcaga
      241 gagtgtgtgg ggtgatgaga aatcttcctt catctgcaac acactccagc ctggctgcaa
      301 cagcgtttgc tatgaccaat tcttccccat ctcccatgtg cggctgtggt ccctgcagct
      361 catcctagtt tccaccccag ctctcctcgt ggccatgcac gtggctcacc agcaacacat
      421 agagaagaaa atgctacggc ttgagggcca tggggacccc ctacacctgg aggaggtgaa
      481 gaggcacaag gtccacatct cagggacact gtggtggacc tatgtcatca gcgtggtgtt
      541 ccggctgttg tttgaggccg tcttcatgta tgtcttttat ctgctctacc ctggctatgc
      601 catggtgcgg ctggtcaagt gcgacgtcta cccctgcccc aacacagtgg actgcttcgt
      661 gtcccgcccc accgagaaaa ccgtcttcac cgtcttcatg ctagctgcct ctggcatctg
      721 catcatcctc aatgtggccg aggtggtgta cctcatcatc cgggcctgtg cccgccgagc
      781 ccagcgccgc tccaatccac cttcccgcaa gggctcgggc ttcggccacc gcctctcacc
      841 tgaatacaag cagaatgaga tcaacaagct gctgagtgag caggatggct ccctgaaaga
      901 catactgcgc cgcagccctg gcaccggggc tgggctggct gaaaagagcg accgctgctc
      961 ggcctgcgcg gatccaccgg tcgccaccat ggtgagtgtg attaaaccag agatgaagat
     1021 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac
     1081 aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg ccgagggcgg
     1141 gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagagtatt
     1201 tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc
     1261 atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag
     1321 ccttagagga aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga
     1381 tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc
     1441 cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa
     1501 tcacaaatgc caaatgaaga ctacttacaa ggcggcaaaa gagattcttg aaatgccagg
     1561 agaccattac atcggccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagcaggt
     1621 agaagatgca gtagctcata gctaagcggc cgcgactcta gatcataatc agccatacca
     1681 cattgaga
//
