LOCUS       sequence-89019-e        2109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mKO2-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1407..1416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1413..2069
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcggca
       61 gcacttttct ttcattgggg ggaaggcgtg aggaaagtac caaacagcag cagactttta
      121 aactttaaac agacaggtct gagagcctga actctcattt ttcctttgac ttcagcctcc
      181 aaggagttcc accaactttg gcgcgccggc ttcactttca ttaagtgaaa gagaggtgcc
      241 cagacatggg tgactggagt gccttgggga agcttctgga caaggtccaa gcctactcca
      301 ccgctggagg gaaggtgtgg ctgtcagtgc tcttcatatt cagaatcctg ctcctgggga
      361 cagctgttga gtcagcttgg ggtgatgaac agtctgcctt tcgctgtaac actcaacaac
      421 ctggctgcga aaacgtctgc tatgacaagt ccttccccat ctctcacgtg cgcttctggg
      481 tccttcagat catattcgtg tctgtgccca cactcctgta cttggcccat gtgttctatg
      541 tgatgaggaa ggaagagaag ctaaacaaga aagaagagga gctcaaagtg gcccagactg
      601 acggggtcaa cgtggagatg cacctgaagc agattgaaat caagaagttc aagtacggga
      661 ttgaagagca cggcaaggtg aaaatgaggg gcggcttgct gagaacctac atcatcagca
      721 tcctcttcaa gtctgtcttc gaggtggcct tcctgctcat ccagtggtac atctatgggt
      781 tcagcttgag cgcggtctac acctgcaaga gagatccctg cccccaccag gtagactgct
      841 tcctctcacg tcccacggag aaaaccatct tcatcatctt catgctggtg gtgtccttgg
      901 tgtctctcgc tttgaacatc attgagctct tctacgtctt cttcaaaggc gttaaggatc
      961 gcgtgaaggg aagaagcgat ccttaccacg ccaccactgg cccactgagc ccatcaaaag
     1021 actgcggatc tccaaaatac gcctacttca atggctgctc ctcaccaacg gctccactct
     1081 cgcctatgtc tcctcctggg tacaagctgg ttactggtga cagaaacaat tcctcgtgcc
     1141 gcaattacaa caagcaagct agcgagcaaa actgggcgaa ctacagcgca gagcaaaatc
     1201 gcatggggca ggccggaagc accatctcca actcgcacgc ccagccgttc gatttccccg
     1261 acgacaacca gaatgccaaa aaagttgctg ctggacatga actccagcca ttagccatcg
     1321 tggaccaacg accttccagc agagccagca gccgcgccag cagcaggcct cggcctgatg
     1381 acctggagat tgcggatcca ccggtcgcca ccatggtgag tgtgattaaa ccagagatga
     1441 agatgaggta ctacatggac ggctccgtca atgggcatga gttcacaatt gaaggtgaag
     1501 gcacaggcag accttacgag ggacatcaag agatgacact acgcgtcaca atggccgagg
     1561 gcgggccaat gcctttcgcg tttgacttag tgtcacacgt gttctgttac ggccacagag
     1621 tatttactaa atatccagaa gagataccag actatttcaa acaagcattt cctgaaggcc
     1681 tgtcatggga aaggtcgttg gagttcgaag atggtgggtc cgcttcagtc agtgcgcata
     1741 taagccttag aggaaacacc ttctaccaca aatccaaatt tactggggtt aactttcctg
     1801 ccgatggtcc tatcatgcaa aaccaaagtg ttgattggga gccatcaacc gagaaaatta
     1861 ctgccagcga cggagttctg aagggtgatg ttacgatgta cctaaaactt gaaggaggcg
     1921 gcaatcacaa atgccaaatg aagactactt acaaggcggc aaaagagatt cttgaaatgc
     1981 caggagacca ttacatcggc catcgcctcg tcaggaaaac cgaaggcaac attactgagc
     2041 aggtagaaga tgcagtagct catagctaag cggccgcgac tctagatcat aatcagccat
     2101 accacattg
//
