LOCUS       sequence-89020-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      239..248
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             245..901
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga
       61 tctggtaccc cttgcagctt ccagttgtca ggaagagaag gctcccatgg atcctgtggt
      121 ggtgctgggg ctctgtctct cctgtttgct tctcctttca ctctggaaac agagctatgg
      181 gggagggaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc
      241 caccatggtg agtgtgatta aaccagagat gaagatgagg tactacatgg acggctccgt
      301 caatgggcat gagttcacaa ttgaaggtga aggcacaggc agaccttacg agggacatca
      361 agagatgaca ctacgcgtca caatggccga gggcgggcca atgcctttcg cgtttgactt
      421 agtgtcacac gtgttctgtt acggccacag agtatttact aaatatccag aagagatacc
      481 agactatttc aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt tggagttcga
      541 agatggtggg tccgcttcag tcagtgcgca tataagcctt agaggaaaca ccttctacca
      601 caaatccaaa tttactgggg ttaactttcc tgccgatggt cctatcatgc aaaaccaaag
      661 tgttgattgg gagccatcaa ccgagaaaat tactgccagc gacggagttc tgaagggtga
      721 tgttacgatg tacctaaaac ttgaaggagg cggcaatcac aaatgccaaa tgaagactac
      781 ttacaaggcg gcaaaagaga ttcttgaaat gccaggagac cattacatcg gccatcgcct
      841 cgtcaggaaa accgaaggca acattactga gcaggtagaa gatgcagtag ctcatagcta
      901 agcggccgcg actctagatc ataatcagcc ataccacatt gtagaaattt ccc
//
