LOCUS       sequence-89022-e        1467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1467)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..1467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      782..791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             788..1444
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 atccgctagc gccaccatgg aatatcatcc tgatttagaa aatttggatg aagatggata
       61 tactcaatta cacttcgact ctcaaagcaa taccaggata gctgttgttt cagagaaagg
      121 atcgtgtgct gcatctcctc cttggcgcct cattgctgta attttgggaa tcctatgctt
      181 ggtaatactg gtgatagctg tggtcctggg taccatggct atttggagat ccaattcagg
      241 aagcaacaca ttggagaatg gctactttct atcaagaaat aaagagaacc acagtcaacc
      301 cacacaatca tctttagaag acagtgtgac tcctaccaaa gctgtcaaaa ccacaggggt
      361 tctttccagc ccttgtcctc ctaattggat tatatatgag aagagctgtt atctattcag
      421 catgtcacta aattcctggg atggaagtaa aagacaatgc tggcaactgg gctctaatct
      481 cctaaagata gacagctcaa atgaattggg atttatagta aaacaagtgt cttcccaacc
      541 tgataattca ttttggatag gcctttctcg gccccagact gaagtaccat ggctctggga
      601 ggatggatca acattctctt ctaacttatt tcagatcaga accacagcta cccaagaaaa
      661 cccatctcca aattgtgtat ggattcacgt gtcagtcatt tatgaccaac tgtgtagtgt
      721 gccctcatat agtatttgtg agaagaagtt ttcaatgagc tccggagggg atccaccggt
      781 cgccaccatg gtgagtgtga ttaaaccaga gatgaagatg aggtactaca tggacggctc
      841 cgtcaatggg catgagttca caattgaagg tgaaggcaca ggcagacctt acgagggaca
      901 tcaagagatg acactacgcg tcacaatggc cgagggcggg ccaatgcctt tcgcgtttga
      961 cttagtgtca cacgtgttct gttacggcca cagagtattt actaaatatc cagaagagat
     1021 accagactat ttcaaacaag catttcctga aggcctgtca tgggaaaggt cgttggagtt
     1081 cgaagatggt gggtccgctt cagtcagtgc gcatataagc cttagaggaa acaccttcta
     1141 ccacaaatcc aaatttactg gggttaactt tcctgccgat ggtcctatca tgcaaaacca
     1201 aagtgttgat tgggagccat caaccgagaa aattactgcc agcgacggag ttctgaaggg
     1261 tgatgttacg atgtacctaa aacttgaagg aggcggcaat cacaaatgcc aaatgaagac
     1321 tacttacaag gcggcaaaag agattcttga aatgccagga gaccattaca tcggccatcg
     1381 cctcgtcagg aaaaccgaag gcaacattac tgagcaggta gaagatgcag tagctcatag
     1441 ctaagcggcc gcgactctag atcataa
//
