LOCUS       sequence-89024-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-ER-5
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        77..128
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     79..99
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..882
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
       61 caactccgcc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      121 gcagagctgg tttagtgaac cgtcagatcc gctagcgcca ccatgctgct atccgtgccg
      181 ttgctgctcg gcctcctcgg cctggccgtc gccgcaccgg tcgccaccat ggtgagtgtg
      241 attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc
      301 acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc
      361 gtcacaatgg ccgagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc
      421 tgttacggcc acagagtatt tactaaatat ccagaagaga taccagacta tttcaaacaa
      481 gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct
      541 tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc caaatttact
      601 ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca
      661 tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta
      721 aaacttgaag gaggcggcaa tcacaaatgc caaatgaaga ctacttacaa ggcggcaaaa
      781 gagattcttg aaatgccagg agaccattac atcggccatc gcctcgtcag gaaaaccgaa
      841 ggcaacatta ctgagcaggt agaagatgca gtagctcata gctccggaaa ggacgagctg
      901 taagaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
      961 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1021 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaa
//
