LOCUS       sequence-89025-e        1728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1728)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             complement(1484..1495)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggactcaga tctcgagcca tgaagccagg
      721 attcagtccc cgtgggggtg gctttggcgg ccgagggggc tttggtgacc gtggtggtcg
      781 tggaggccga gggggctttg gcgggggccg aggccaaggc ggaggcttta gaggtcgtgg
      841 acgaggagga ggtggaggcg gcggcggcgg tggaggagga ggaagaggtg gtggaggctt
      901 ccattttggt ggcaaccggg gtcgtggtcg gggaggaaaa agaggaaacc agtcggggaa
      961 gaatgtgatg gtggagccgc atcggcatga gggtgtcttc atttgtcggg gaaaggaaga
     1021 tgcactggtc accaagaacc tggtccctgg ggaatcagtt tatggagaga agagagtctc
     1081 gatttcggaa ggagatgaca aaattgagta ccgagcctgg aaccccttcc gctccaagct
     1141 tgcagcagca atcctgggtg gtgtggacca gatccacatc aaaccggggg ctaaggttct
     1201 ctacctcggg gctgcctcgg gcaccacggt ctcccatgtc tctgacatcg ttggtccgga
     1261 tggtctagtc tatgcagtcg agttctccca ccgctctggc cgtgacctca ttaacttggc
     1321 caagaagagg accaacatca ttcctgtgat cgaggatgct cgacacccac acaaataccg
     1381 catgctcatc gcaatggtgg atgtgatctt tgctgatgtg gcccagccag accagacccg
     1441 gattgtggcc ctgaatgccc acaccttcct gcgtaatgga ggacactttg tgatttccat
     1501 taaggccaac tgcattgact ccacagcctc agccgaggcc gtgtttgcct ccgaagtgaa
     1561 aaagatgcaa caggagaaca tgaagccgca ggagcagttg acccttgagc catatgaaag
     1621 agaccatgcc gtggtcgtgg gagtgtacag gccacccccc aaggtgaaga actgatgagg
     1681 atccaccgga tctagataac tgatcataat cagccatacc acattgag
//
