LOCUS       sequence-89026-e        2199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 551, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2199)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-FYN-N-10
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1497..1506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1503..2159
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ggacggtcag atccgctagc gccaccatgg gctgtgtgca atgtaaggat aaagaagcaa
       61 caaaactgac ggaggagagg gacggcagcc tgaaccagag ctctgggtac cgctatggca
      121 cagaccccac ccctcagcac taccccagct tcggtgtgac ctccatcccc aactacaaca
      181 acttccacgc agccgggggc caaggactca ccgtctttgg aggtgtgaac tcttcgtctc
      241 atacggggac cttgcgtacg agaggaggaa caggagtgac actctttgtg gccctttatg
      301 actatgaagc acggacagaa gatgacctga gttttcacaa aggagaaaaa tttcaaatat
      361 tgaacagctc ggaaggagat tggtgggaag cccgctcctt gacaactgga gagacaggtt
      421 acattcccag caattatgtg gctccagttg actctatcca ggcagaagag tggtactttg
      481 gaaaacttgg ccgaaaagat gctgagcgac agctattgtc ctttggaaac ccaagaggta
      541 cctttcttat ccgcgagagt gaaaccacca aaggtgccta ttcactttct atccgtgatt
      601 gggatgatat gaaaggagac catgtcaaac attataaaat tcgcaaactt gacaatggtg
      661 gatactacat taccacccgg gcccagtttg aaacacttca gcagcttgta caacattact
      721 caggtacctg gaatggaaac acaaaagtag ccataaagac tcttaaacca ggcacaatgt
      781 cccccgaatc attccttgag gaagcgcaga tcatgaagaa gctgaagcac gacaagctgg
      841 tccagctcta tgcagtggtg tctgaggagc ccatctacat cgtcaccgag tatatgaaca
      901 aaggaagttt actggatttc ttaaaagatg gagaaggaag agctctgaaa ttaccaaatc
      961 ttgtggacat ggcagcacag gtggctgcag gaatggctta catcgagcgc atgaattata
     1021 tccatagaga tctgcgatca gcaaacattc tagtggggaa tggactcata tgcaagattg
     1081 ctgacttcgg attggcccga ttgatagaag acaatgagta cacagcaaga caaggtgcaa
     1141 agttccccat caagtggacg gcccccgagg cagccctgta cgggaggttc acaatcaagt
     1201 ctgacgtgtg gtcttttgga atcttactca cagagctggt caccaaagga agagtgccat
     1261 acccaggcat gaacaaccgg gaggtgctgg agcaggtgga gcgaggctac aggatgccct
     1321 gcccgcagga ctgccccatc tctctgcatg agctcatgat ccactgctgg aaaaaggacc
     1381 ctgaagaacg ccccactttt gagtacttgc agagcttcct ggaagactac tttaccgcga
     1441 cagagcccca gtaccaacct ggtgaaaacc tggcatccag cggggatcca ccggtcgcca
     1501 ccatggtgag tgtgattaaa ccagagatga agatgaggta ctacatggac ggctccgtca
     1561 atgggcatga gttcacaatt gaaggtgaag gcacaggcag accttacgag ggacatcaag
     1621 agatgacact acgcgtcaca atggccgagg gcgggccaat gcctttcgcg tttgacttag
     1681 tgtcacacgt gttctgttac ggccacagag tatttactaa atatccagaa gagataccag
     1741 actatttcaa acaagcattt cctgaaggcc tgtcatggga aaggtcgttg gagttcgaag
     1801 atggtgggtc cgcttcagtc agtgcgcata taagccttag aggaaacacc ttctaccaca
     1861 aatccaaatt tactggggtt aactttcctg ccgatggtcc tatcatgcaa aaccaaagtg
     1921 ttgattggga gccatcaacc gagaaaatta ctgccagcga cggagttctg aagggtgatg
     1981 ttacgatgta cctaaaactt gaaggaggcg gcaatcacaa atgccaaatg aagactactt
     2041 acaaggcggc aaaagagatt cttgaaatgc caggagacca ttacatcggc catcgcctcg
     2101 tcaggaaaac cgaaggcaac attactgagc aggtagaaga tgcagtagct catagctaag
     2161 cggccgcgac tctagatcat aatcagccat accacattt
//
