LOCUS       sequence-89027-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Golgi-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..957
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcatgaggc ttcgggagcc gctcctgagc
       61 ggcagcgccg cgatgccagg cgcgtcccta cagcgggcct gccgcctgct cgtggccgtc
      121 tgcgctctgc accttggcgt caccctcgtt tactacctgg ctggccgcga cctgagccgc
      181 ctgccccaac tggtcggagt ctccacaccg ctgcagggcg gctcgaacag tgccgccgcc
      241 atcgggcagt cctccgggga gctccggacc ggaggggcca aggatccacc ggtcgccacc
      301 atggtgagtg tgattaaacc agagatgaag atgaggtact acatggacgg ctccgtcaat
      361 gggcatgagt tcacaattga aggtgaaggc acaggcagac cttacgaggg acatcaagag
      421 atgacactac gcgtcacaat ggccgagggc gggccaatgc ctttcgcgtt tgacttagtg
      481 tcacacgtgt tctgttacgg ccacagagta tttactaaat atccagaaga gataccagac
      541 tatttcaaac aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat
      601 ggtgggtccg cttcagtcag tgcgcatata agccttagag gaaacacctt ctaccacaaa
      661 tccaaattta ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt
      721 gattgggagc catcaaccga gaaaattact gccagcgacg gagttctgaa gggtgatgtt
      781 acgatgtacc taaaacttga aggaggcggc aatcacaaat gccaaatgaa gactacttac
      841 aaggcggcaa aagagattct tgaaatgcca ggagaccatt acatcggcca tcgcctcgtc
      901 aggaaaaccg aaggcaacat tactgagcag gtagaagatg cagtagctca tagctaagcg
      961 gccgcgactc tagatcataa tcagccatac caca
//
