LOCUS       sequence-89028-e        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-H2B-6
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             475..1131
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agtgtgatta aaccagagat gaagatgagg tactacatgg acggctccgt caatgggcat
      541 gagttcacaa ttgaaggtga aggcacaggc agaccttacg agggacatca agagatgaca
      601 ctacgcgtca caatggccga gggcgggcca atgcctttcg cgtttgactt agtgtcacac
      661 gtgttctgtt acggccacag agtatttact aaatatccag aagagatacc agactatttc
      721 aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt tggagttcga agatggtggg
      781 tccgcttcag tcagtgcgca tataagcctt agaggaaaca ccttctacca caaatccaaa
      841 tttactgggg ttaactttcc tgccgatggt cctatcatgc aaaaccaaag tgttgattgg
      901 gagccatcaa ccgagaaaat tactgccagc gacggagttc tgaagggtga tgttacgatg
      961 tacctaaaac ttgaaggagg cggcaatcac aaatgccaaa tgaagactac ttacaaggcg
     1021 gcaaaagaga ttcttgaaat gccaggagac cattacatcg gccatcgcct cgtcaggaaa
     1081 accgaaggca acattactga gcaggtagaa gatgcagtag ctcatagcta agcggccgcg
     1141 actctagatc ataatcagcc ataccacatt gag
//
