LOCUS       sequence-89033-e        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      389..398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             395..1051
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctgtgacgtc agatccgcta gcgccaccat gaagttaagt cgccagttca ccgtgtttgg
       61 cagcgcgatc ttctgcgtcg taatcttctc actctacctg atgctggaca ggggtcactt
      121 ggactaccct cggggcccgc gccaggaggg ctcctttccg cagggccagc tttcaatatt
      181 gcaagaaaag attgaccatt tggagcgttt gctcgctgag aacaacgaga tcatctcaaa
      241 tatcagagac tcagtcatca acctgagcga gtctgtggag gacggcccgc gggggtcacc
      301 aggcaacgcc agccaaggct ccatccacct ccactcgcca cagttggccc tgcaggctga
      361 ccccagctcc ggatcggatc caccggtcgc caccatggtg agtgtgatta aaccagagat
      421 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      481 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      541 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      601 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      661 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      721 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      781 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      841 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      901 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      961 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
     1021 gcaggtagaa gatgcagtag ctcatagcta agcggccgcg actctagatc ataatcagcc
     1081 ataccacat
//
