LOCUS       sequence-89034-e        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..690
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagtgtgat taaaccagag
       61 atgaagatga ggtactacat ggacggctcc gtcaatgggc atgagttcac aattgaaggt
      121 gaaggcacag gcagacctta cgagggacat caagagatga cactacgcgt cacaatggcc
      181 gagggcgggc caatgccttt cgcgtttgac ttagtgtcac acgtgttctg ttacggccac
      241 agagtattta ctaaatatcc agaagagata ccagactatt tcaaacaagc atttcctgaa
      301 ggcctgtcat gggaaaggtc gttggagttc gaagatggtg ggtccgcttc agtcagtgcg
      361 catataagcc ttagaggaaa caccttctac cacaaatcca aatttactgg ggttaacttt
      421 cctgccgatg gtcctatcat gcaaaaccaa agtgttgatt gggagccatc aaccgagaaa
      481 attactgcca gcgacggagt tctgaagggt gatgttacga tgtacctaaa acttgaagga
      541 ggcggcaatc acaaatgcca aatgaagact acttacaagg cggcaaaaga gattcttgaa
      601 atgccaggag accattacat cggccatcgc ctcgtcagga aaaccgaagg caacattact
      661 gagcaggtag aagatgcagt agctcatagc tccggactca gatctggtgg ctccgcagga
      721 tcccggcaag aagaagcaaa ggctgctgta ggtgtgactg gaaatgacat cactaccccg
      781 ccaaacaagg agccaccacc aagcccagaa aagaaagcaa agcctttggc caccactcaa
      841 cctgcaaaga cttcaacatc gaaagccaaa acacagccca cttctctccc taagcaacca
      901 gctcccacca cctctggtgg gttgaataaa aaacccatga gcctcgcctc aggctcagtg
      961 ccagctgccc cacacaaacg ccctgctgct gccactgcta ctgccaggcc ttccacccta
     1021 cctgccagag acgtgaagcc aaagccaatt acagaagcta aggttgccga aaagcggacc
     1081 tctccatcca agccttcatc tgccccagcc ctcaaacctg gacctaaaac caccccaacc
     1141 gtttcaaaag ccacatctcc ctcaactctt gtttccactg gaccaagtag tagaagtcca
     1201 gctacaactc tgcctaagag gccaaccagc atcaagactg aggggaaacc tgctgatgtc
     1261 aaaaggatga ctgctaagtc tgcctcagct gacttgagtc gctcaaagac cacctctgcc
     1321 agttctgtga agagaaacac cactcccact ggggcagcac ccccagcagg gatgacttcc
     1381 actcgagtca agcccatgtc tgcacctagc cgctcttctg gggctctttc tgtggacaag
     1441 aagcccactt ccactaagcc tagctcctct gctcccaggg tgagccgcct ggccacaact
     1501 gtttctgccc ctgacctgaa gagtgttcgc tccaaggtcg gctctacaga aaacatcaaa
     1561 caccagcctg gaggaggccg ggccaaggta gagaaaaaaa cagaggcagc taccacagct
     1621 gggaagcctg aacctaatgc agtcactaaa gcagccggct ccattgcgag tgcacagaaa
     1681 ccgcctgctg ggaaagtcca gatagtatcc aaaaaagtga gctacagtca tattcaatcc
     1741 aagtgtgttt ccaaggacaa tattaagcat gtccctggat gtggcaatgt tcagattcag
     1801 aacaagaaag tggacatatc caaggtctcc tccaagtgtg ggtccaaagc taatatcaag
     1861 cacaagcctg gtggaggaga tgtcaagatt gaaagtcaga agttgaactt caaggagaag
     1921 gcccaagcca aagtgggata gggatccacc ggatctagat aactgatcat aatcagccat
     1981 accacatt
//
