LOCUS       sequence-89035-e        1820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1820)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg
       61 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      121 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      181 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      241 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      301 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      361 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      421 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      481 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      541 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      601 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      661 caggtagaag atgcagtagc tcatagctcc ggaagcggcg ggtccgggag cggaagcgct
      721 gagccccgcc aggagttcga agtgatggaa gatcacgctg ggacgtacgg gttgggggac
      781 aggaaagatc aggggggcta caccatgcac caagaccaag agggtgacac ggacgctggc
      841 ctgaaagctg aagaagcagg cattggagac acccccagcc tggaagacga agctgctggt
      901 cacgtgaccc aagctcgcat ggtcagtaaa agcaaagacg ggactggaag cgatgacaaa
      961 aaagccaagg gggctgatgg taaaacgaag atcgccacac cgcggggagc agcccctcca
     1021 ggccagaagg gccaggccaa cgccaccagg attccagcaa aaaccccgcc cgctccaaag
     1081 acaccaccca gctctggtga acctccaaaa tcaggggatc gcagcggcta cagcagcccc
     1141 ggctccccag gcactcccgg cagccgctcc cgcaccccgt cccttccaac cccacccacc
     1201 cgggagccca agaaggtggc agtggtccgt actccaccca agtcgccgtc ttccgccaag
     1261 agccgcctgc agacagcccc cgtgcccatg ccagacctga agaatgtcaa gtccaagatc
     1321 ggctccactg agaacctgaa gcaccagccg ggaggcggga aggtgcaaat agtctacaaa
     1381 ccagttgacc tgagcaaggt gacctccaag tgtggctcat taggcaacat ccatcataaa
     1441 ccaggaggtg gccaggtgga agtaaaatct gagaagcttg acttcaagga cagagtccag
     1501 tcgaagattg ggtccctgga caatatcacc cacgtccctg gcggaggaaa taaaaagatt
     1561 gaaacccaca agctgacctt ccgcgagaac gccaaagcca agacagacca cggggcggag
     1621 atcgtgtaca agtcgccagt ggtgtctggg gacacgtctc cacggcatct cagcaatgtc
     1681 tcctccaccg gcagcatcga catggtagac tcgccccagc tcgccacgct agctgacgag
     1741 gtgtctgcct ccctggccaa gcagggtttg tgaggatcca ccggatctag ataactgatc
     1801 ataatcagcc ataccacatt
//
