LOCUS       sequence-89036-e        1809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1809)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1108..1117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1114..1770
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggat ctgcaagcct cgttgctgtc cactggcccc
       61 aatgccagca acatctccga tggccaggat aatttcacat tggcggggcc acctcctcgc
      121 acaaggagtg tctcctacat caacatcatc atgccttcag tgtttggtac catctgtctc
      181 ctgggcattg tgggaaactc cacagtcatt tttgccgtgg tgaagaaatc caagctgcac
      241 tggtgcagca acgtccctga catcttcatc atcaacctct ctgtggtgga tctgcttttc
      301 ctgctgggca tgcctttcat gatccaccag ctcatgggta atggtgtctg gcactttggg
      361 gaaaccatgt gcaccctcat cacagccatg gacgccaaca gtcagttcac cagcacctac
      421 atcctgactg ctatggccat tgaccgctac ttggccaccg tccatcccat ctcctccacc
      481 aagttccgga agccctccat ggccaccctg gtgatctgcc tcctgtgggc tctctcgttc
      541 attagcatca ctcctgtgtg gctctatgcc aggcttatcc ccttcccagg gggtgctgtg
      601 ggctgtggca tccgcctacc aaacccagat actgatcttt actggttcac tctgtatcag
      661 tttttcctgg ccttcgccct tccgtttgtg gtcatcactg ctgcgtacgt gaaaatacta
      721 cagcgcatga cgtcttcggt ggccccagcc tctcaacgca gcatccggct tcggacaaag
      781 agggtgaccc gcacagccat tgccatctgt ctggtcttct ttgtgtgctg ggcgccctac
      841 tacgtgctgc agctgaccca gttgtccatc agccgcccga ccctcacatt cgtctacctg
      901 tacaatgcgg ctatcagctt gggctatgcc aacagctgcc tcaatccctt tgtgtacata
      961 gtactctgtg agacctttcg aaaacgcttg gtgctgtcgg tgaagcccgc ggcccagggg
     1021 cagcttcgca cggtcagcaa tgctcagaca gctgacgagg agaggacaga aagcaaaggc
     1081 accggaggta gcggggatcc accggtcgcc accatggtga gtgtgattaa accagagatg
     1141 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
     1201 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
     1261 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
     1321 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
     1381 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
     1441 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
     1501 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
     1561 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
     1621 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
     1681 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
     1741 caggtagaag atgcagtagc tcatagctaa gcggccgcga ctctagatca taatcagcca
     1801 taccacatt
//
