LOCUS       sequence-89037-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Mito-7
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             84..158
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..848
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 catgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga
      121 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac
      181 cggtcgccac catggtgagt gtgattaaac cagagatgaa gatgaggtac tacatggacg
      241 gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg cacaggcaga ccttacgagg
      301 gacatcaaga gatgacacta cgcgtcacaa tggccgaggg cgggccaatg cctttcgcgt
      361 ttgacttagt gtcacacgtg ttctgttacg gccacagagt atttactaaa tatccagaag
      421 agataccaga ctatttcaaa caagcatttc ctgaaggcct gtcatgggaa aggtcgttgg
      481 agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat aagccttaga ggaaacacct
      541 tctaccacaa atccaaattt actggggtta actttcctgc cgatggtcct atcatgcaaa
      601 accaaagtgt tgattgggag ccatcaaccg agaaaattac tgccagcgac ggagttctga
      661 agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg caatcacaaa tgccaaatga
      721 agactactta caaggcggca aaagagattc ttgaaatgcc aggagaccat tacatcggcc
      781 atcgcctcgt caggaaaacc gaaggcaaca ttactgagca ggtagaagat gcagtagctc
      841 atagctaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
      901 cttgctttaa aaaacctccc aca
//
