LOCUS       sequence-89039-e        2260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2260)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..677
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg aagatgaggt
       61 actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa ggcacaggca
      121 gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag ggcgggccaa
      181 tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga gtatttacta
      241 aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc ctgtcatggg
      301 aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat ataagcctta
      361 gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct gccgatggtc
      421 ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt actgccagcg
      481 acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc ggcaatcaca
      541 aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg ccaggagacc
      601 attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag caggtagaag
      661 atgcagtagc tcatagctcc ggaagtgctg ggagcgcaag cggaagcgga ggctccggtt
      721 ttaactacga acgtccaaaa cacttcatcc agtcccaaaa cccatgtggc tccagattgc
      781 agcctcctgg accagaaacc tccagcttct ctagccagac caaacagtct tccattatca
      841 tccagccccg ccagtgtaca gagcaaagat tttctgcctc ctcaacactg agctctcaca
      901 tcaccatgtc ctcctctgct ttccctgctt ctccccagca gcatgctggc tccaacccag
      961 gccaaagggt tacaaccacc tataaccagt ccccagccag cttcctcagc tccatattac
     1021 catcacagcc tgattacaat agcagtaaaa tcccttccgc tatggattcc aactatcaac
     1081 agtcctcagc tggccaacct ataaatgcaa agccatccca aactgcaaat gctaagccca
     1141 taccaagaac tcctgatcat gaaatacaag gatcaaaaga agctttgatt caagatttgg
     1201 aaagaaagct gaaatgcaag gacacccttc ttcataatgg aaatcaacgt ctaacatatg
     1261 aagagaagat ggctcgcaga ttgctaggac cacagaatgc agctgctgtg tttcaagctc
     1321 aggatgacag tggtgcacaa gactcgcagc aacacaactc agaacatgcg cgactgcaag
     1381 ttcctacatc acaagtaaga agtagatcaa cctcaagggg agatgtgaat gatcaggatg
     1441 caatccagga gaaattttac ccaccacgtt tcattcaagt gccagagaac atgtcgattg
     1501 atgaaggaag attctgcaga atggacttca aagtgagtgg actgccagct cctgatgtgt
     1561 catggtatct aaatggaaga acagttcaat cagatgattt gcacaaaatg atagtgtctg
     1621 agaagggtct tcattcactc atctttgaag tagtcagagc ttcagatgca ggggcttatg
     1681 catgtgttgc caagaataga gcaggagaag ccaccttcac tgtgcagctg gatgtccttg
     1741 caaaagaaca taaaagagca ccaatgttta tctacaaacc acagagcaaa aaagttttag
     1801 agggagattc agtgaaacta gaatgccaga tctcggctat acctccacca aagcttttct
     1861 ggaaaagaaa taatgaaatg gtacaattca acactgaccg aataagctta tatcaagata
     1921 acactggaag agttacttta ctgataaaag atgtaaacaa gaaagatgct gggtggtata
     1981 ctgtgtcagc agttaatgaa gctggagtga ctacatgtaa cacaagatta gacgttacgg
     2041 cacgtccaaa ccaaactctt ccagctccta agcagttacg ggttcgacca acattcagca
     2101 aatatttagc acttaatggg aaaggtttga atgtaaaaca agcttttaac ccagaaggag
     2161 aatttcagcg tttggcagct caatctggac tctatgaaag tgaagaactt taaggatcca
     2221 ccggatctag ataactgatc ataatcagcc ataccacatt
//
