LOCUS       sequence-89040-e        2776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2776)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggactcaga tctcgagtga gcgctctggc
      721 ctcccgcgct accgccatgc agtcctccgg ctccttcaac tacgcgcgcc ctaagcagtt
      781 catcgcggcg cagaacctgg gtcccgcatc ggggctgccc acgccaacct ccagccccag
      841 ctcctccagc ttgccgtcac cattgtcccc cacgcccagg cccttcggcc gcgcgcccgg
      901 gccgcccttc gtggaacccg aggccatgtg gggcccgtcc tcgccctcgc cgccaccgcc
      961 gccacctccg gtcttcagcc cctctgcagc ctacccggtg cccgacgtgt tcccactgcc
     1021 accgccgccg ccaccgctac ctagctccac ctcacactgc gcctcgcccg cccgcttcgg
     1081 ccccagccag acgcccgcag ccttcctcag cgccctgctg ccctctcagc caccgcctgt
     1141 cgctgtcaac gccctggggc tgcccaaggg agtcaccccc gcggggttcc caaagaagtc
     1201 cagtagaact gctagaattg cctctgatga ggagattcaa ggcacaaagg atgctgtcat
     1261 ccaagacctg gaacggaagc ttcgcttcaa ggaggacctt ctgaacaatg gccaaccgag
     1321 gctaacctat gaggaaagaa tggctcgccg cctgcttgga gccgacagcg caaacgtctt
     1381 caacatccag gagccagagg aaacggcagc caatcaggag tacaaagtct ctagctgcga
     1441 gcagaggctg attagcgaga ttgaatacag gctggagcgc tcccctgtgg atgagtcggg
     1501 agacgaggtg caggatccag atgtgcctgt ggagaacgca acagctccct tctttgagat
     1561 gaagctgaaa cactacaaga tctttgaggg gatgccggtg actttcacgt gtcgagtggc
     1621 tgggaatcca aagccaaaga tctattggtt taaagatggg aagcagattt ctccgaagag
     1681 cgatcactac accattcaga gagaccttga tgggacctgc tctctccaca ccacggcctc
     1741 taccctagac gacgatggga actacaccat catggctgcc aaccctcagg gtcgcgtcag
     1801 ttgtacagga aggctaatgg tacaggctgt caaccaaaga ggccgcagtc cccgctctcc
     1861 ctcaggccat cctcatgcca gaaggcctcg ctctcgatca cgggacagtg gagatgaaaa
     1921 cgagcccatt caggagcgat tcttcagacc tcacttcctg caggctcctg gagacctgac
     1981 cgttcaggaa ggcaagctct gcaggatgga ctgcaaggtc agtgggttac caaccccaga
     2041 tctcagctgg caactagacg gaaagcccat acgccccgac agtgctcaca agatgctggt
     2101 ccgtgagaat ggggtccact ccctcattat agagccagtc acgtcccggg acgccggcat
     2161 ctacacatgt attgccacca acagagcagg acagaactcg tttaacctgg agcttgtggt
     2221 tgctgctaag gaagcacaca aggcccctgt gtttatggag aagctacaga acacgggggt
     2281 tgctgatgga tacccagtgc ggctagaatg ccgtgtctcc ggagtgccgc cacctcagat
     2341 attttggaag aaagaaaatg aatcgctcac tcatagcact gagcgagtaa gcatgcacca
     2401 ggataatcat ggctacatct gcctgctcat ccagggagcc acaaaggaag acgctgggtg
     2461 gtacactgtg tctgccaaga acgaagcagg cattgtgtcc tgcactgccc ggctggatgt
     2521 ctacacccag tggcaccagc agccacagac caccaagcca aaaaaagtac ggccctcggc
     2581 cagtcgctac gcagcacttt cggaccaggg actagacatc aaagccgctt tccaacctga
     2641 agccagccca tctcacctga ctctgaacag cggcttggta gaaagtgaag acctgtgaga
     2701 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
     2761 tcagccatac cacatt
//
