LOCUS       sequence-89041-e        1654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1654)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..50
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             78..98
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..764
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 cgacgtcaga tccgctagcc accatggatc caaaaaagaa gagaaaggta gatccaaaaa
       61 agaagagaaa ggtagatcca aaaaagaaga gaaaggtacc ggtcgccacc atggtgagtg
      121 tgattaaacc agagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt
      181 tcacaattga aggtgaaggc acaggcagac cttacgaggg acatcaagag atgacactac
      241 gcgtcacaat ggccgagggc gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt
      301 tctgttacgg ccacagagta tttactaaat atccagaaga gataccagac tatttcaaac
      361 aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg
      421 cttcagtcag tgcgcatata agccttagag gaaacacctt ctaccacaaa tccaaattta
      481 ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc
      541 catcaaccga gaaaattact gccagcgacg gagttctgaa gggtgatgtt acgatgtacc
      601 taaaacttga aggaggcggc aatcacaaat gccaaatgaa gactacttac aaggcggcaa
      661 aagagattct tgaaatgcca ggagaccatt acatcggcca tcgcctcgtc aggaaaaccg
      721 aaggcaacat tactgagcag gtagaagatg cagtagctca tagctccgga gaagggcaag
      781 ggcaagggca agggccgggc cgcggctacg cgtatcgatc catgttcgag gcgcgcctgg
      841 tccagggctc catcctcaag aaggtgttgg aggcactcaa ggacctcatc aacgaggcct
      901 gctgggatat tagctccagc ggtgtaaacc tgcagagcat ggactcgtcc cacgtctctt
      961 tggtgcagct caccctgcgg tctgagggct tcgacaccta ccgctgcgac cgcaacctgg
     1021 ccatgggcgt gaacctcacc agtatgtcca aaatactaaa atgcgccggc aatgaagata
     1081 tcattacact aagggccgaa gataacgcgg ataccttggc gctagtattt gaagcaccaa
     1141 accaggagaa agtttcagac tatgaaatga agttgatgga tttagatgtt gaacaacttg
     1201 gaattccaga acaggagtac agctgtgtag taaagatgcc ttctggtgaa tttgcacgta
     1261 tatgccgaga tctcagccat attggagatg ctgttgtaat ttcctgtgca aaagacggag
     1321 tgaaattttc tgcaagtgga gaacttggaa atggaaacat taaattgtca cagacaagta
     1381 atgtcgataa agaggaggaa gctgttacca tagagatgaa tgaaccagtt caactaactt
     1441 ttgcactgag gtacctgaac ttctttacaa aagccactcc actctcttca acggtgacac
     1501 tcagtatgtc tgcagatgta ccccttgttg tagagtataa aattgcggat atgggacact
     1561 taaaatacta cttggctccc aagatcgagg atgaagaagg atcttaggga tccaccggat
     1621 ctagataact gatcataatc agccatacca catt
//
