LOCUS       sequence-89042-e        2585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2585)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..2585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1876..1885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1882..2538
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgagt gctgcagtga ctgcagggaa gctggcacgg
       61 gcaccggccg accctgggaa agccggggtc cccggagttg cagctcccgg agctccggcg
      121 gcggctccac cggcgaaaga gatcccggag gtcctagtgg acccacgcag ccggcggcgc
      181 tatgtgcggg gccgcttttt gggcaagggc ggctttgcca agtgcttcga gatctcggac
      241 gcggacacca aggaggtgtt cgcgggcaag attgtgccta agtctctgct gctcaagccg
      301 caccagaggg agaagatgtc catggaaata tccattcacc gcagcctcgc ccaccagcac
      361 gtcgtaggat tccacggctt tttcgaggac aacgacttcg tgttcgtggt gttggagctc
      421 tgccgccgga ggtctctcct ggagctgcac aagaggagga aagccctgac tgagcctgag
      481 gcccgatact acctacggca aattgtgctt ggctgccagt acctgcaccg aaaccgagtt
      541 attcatcgag acctcaagct gggcaacctt ttcctgaatg aagatctgga ggtgaaaata
      601 ggggattttg gactggcaac caaagtcgaa tatgacgggg agaggaagaa gaccctgtgt
      661 gggactccta attacatagc tcccgaggtg ctgagcaaga aagggcacag tttcgaggtg
      721 gatgtgtggt ccattgggtg tatcatgtat accttgttag tgggcaaacc accttttgag
      781 acttcttgcc taaaagagac ctacctccgg atcaagaaga atgaatacag tattcccaag
      841 cacatcaacc ccgtggccgc ctccctcatc cagaagatgc ttcagacaga tcccactgcc
      901 cgcccaacca ttaacgagct gcttaatgac gagttcttta cttctggcta tatccctgcc
      961 cgtctcccca tcacctgcct gaccattcca ccaaggtttt cgattgctcc cagcagcctg
     1021 gaccccagca accggaagcc cctcacagtc ctcaataaag gcttggagaa ccccctgcct
     1081 gagcgtcccc gggaaaaaga agaaccagtg gttcgagaga caggtgaggt ggtcgactgc
     1141 cacctcagtg acatgctgca gcagctgcac agtgtcaatg cctccaagcc ctcggagcgt
     1201 gggctggtca ggcaagagga ggctgaggat cctgcctgca tccccatctt ctgggtcagc
     1261 aagtgggtgg actattcgga caagtacggc cttgggtatc agctctgtga taacagcgtg
     1321 ggggtgctct tcaatgactc aacacgcctc atcctctaca atgatggtga cagcctgcag
     1381 tacatagagc gtgacggcac tgagtcctac ctcaccgtga gttcccatcc caactccttg
     1441 atgaagaaga tcaccctcct taaatatttc cgcaattaca tgagcgagca cttgctgaag
     1501 gcaggtgcca acatcacgcc gcgcgaaggt gatgagctcg cccggctgcc ctacctacgg
     1561 acctggttcc gcacccgcag cgccatcatc ctgcacctca gcaacggcag cgtgcagatc
     1621 aacttcttcc aggatcacac caagctcatc ttgtgcccac tgatggcagc cgtgacctac
     1681 atcgacgaga agcgggactt ccgcacatac cgcctgagtc tcctggagga gtacggctgc
     1741 tgcaaggagc tggccagccg gctccgctac gcccgcacta tggtggacaa gctgctgagc
     1801 tcacgctcgg ccagcaaccg tctcaaggcc tccggtagcg gcagcgcatc cggcggaagc
     1861 ggaagcggac cggtcgccac catggtgagt gtgattaaac cagagatgaa gatgaggtac
     1921 tacatggacg gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg cacaggcaga
     1981 ccttacgagg gacatcaaga gatgacacta cgcgtcacaa tggccgaggg cgggccaatg
     2041 cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg gccacagagt atttactaaa
     2101 tatccagaag agataccaga ctatttcaaa caagcatttc ctgaaggcct gtcatgggaa
     2161 aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat aagccttaga
     2221 ggaaacacct tctaccacaa atccaaattt actggggtta actttcctgc cgatggtcct
     2281 atcatgcaaa accaaagtgt tgattgggag ccatcaaccg agaaaattac tgccagcgac
     2341 ggagttctga agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg caatcacaaa
     2401 tgccaaatga agactactta caaggcggca aaagagattc ttgaaatgcc aggagaccat
     2461 tacatcggcc atcgcctcgt caggaaaacc gaaggcaaca ttactgagca ggtagaagat
     2521 gcagtagctc atagctaagc ggccgcgact ctagatcata atcagccata ccacattgag
     2581 aagtg
//
