LOCUS       sequence-89043-e        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg
       61 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      121 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      181 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      241 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      301 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      361 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      421 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      481 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      541 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      601 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      661 caggtagaag atgcagtagc tcatagctcc ggactcagat ctcgagccat ggctagtcga
      721 ggcgcaacaa gacccaacgg gccaaatact ggaaataaaa tatgccagtt caaactagta
      781 cttctgggag agtctgctgt tggcaaatca agcctagtgc ttcgttttgt gaagggccaa
      841 tttcatgaat ttcaagagag taccataggg gctgcttttc taacccaaac tgtgtgtctt
      901 gatgatacaa cagtaaagtt tgaaatatgg gatacagctg gtcaagaacg ataccatagc
      961 ttagcaccaa tgtactacag aggagcacaa gcagccatag ttgtatatga tatcacaaat
     1021 gaggagtcct ttgccagagc caaaaactgg gttaaagaac ttcagaggca agccagtcct
     1081 aacattgtaa tagctttatc aggaaacaag gctgatcttg caaataaaag agctgtcgat
     1141 ttccaggaag cacagtccta tgcagatgac aacagtttat tattcatgga gacatcagct
     1201 aaaacatcga tgaatgtaaa tgaaatattc atggcaatag ctaaaaagtt gccaaagaac
     1261 gaaccacaga atccaggagc aaattctgcc agaggaagag gagtagacct tactgaaccc
     1321 acgcagccaa ccaggaatca gtgttgtagt aactaaggat ccaccggatc tagataactg
     1381 atcataatca gccataccac att
//
