LOCUS       sequence-89044-e        1372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1372)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..680
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagtgtgat taaaccagag atgaagatga
       61 ggtactacat ggacggctcc gtcaatgggc atgagttcac aattgaaggt gaaggcacag
      121 gcagacctta cgagggacat caagagatga cactacgcgt cacaatggcc gagggcgggc
      181 caatgccttt cgcgtttgac ttagtgtcac acgtgttctg ttacggccac agagtattta
      241 ctaaatatcc agaagagata ccagactatt tcaaacaagc atttcctgaa ggcctgtcat
      301 gggaaaggtc gttggagttc gaagatggtg ggtccgcttc agtcagtgcg catataagcc
      361 ttagaggaaa caccttctac cacaaatcca aatttactgg ggttaacttt cctgccgatg
      421 gtcctatcat gcaaaaccaa agtgttgatt gggagccatc aaccgagaaa attactgcca
      481 gcgacggagt tctgaagggt gatgttacga tgtacctaaa acttgaagga ggcggcaatc
      541 acaaatgcca aatgaagact acttacaagg cggcaaaaga gattcttgaa atgccaggag
      601 accattacat cggccatcgc ctcgtcagga aaaccgaagg caacattact gagcaggtag
      661 aagatgcagt agctcatagc tccggactca gatctcgagc catgacctct aggaagaaag
      721 tgttgctgaa ggttatcatc ctgggagatt ctggagtcgg gaagacatca ctcatgaacc
      781 agtatgtgaa taagaaattc agcaatcagt acaaagccac aataggagct gactttctga
      841 ccaaggaggt gatggtggat gacaggctag tcacaatgca gatatgggac acagcaggac
      901 aggaacggtt ccagtctctc ggtgtggcct tctacagagg tgcagactgc tgcgttctgg
      961 tatttgatgt gactgccccc aacacattca aaaccctaga tagctggaga gatgagtttc
     1021 tcatccaggc cagtccccga gatcctgaaa acttcccatt tgttgtgttg ggaaacaaga
     1081 ttgacctcga aaacagacaa gtggccacaa agcgggcaca ggcctggtgc tacagcaaaa
     1141 acaacattcc ctactttgag accagtgcca aggaggccat caacgtggag caggcgttcc
     1201 agacgattgc acggaatgca cttaagcagg aaacggaggt ggagctgtac aacgaatttc
     1261 ctgaacctat caaactggac aagaatgacc gggccaaggc ctcggcagaa agctgcagtt
     1321 gctgaggatc caccggatct agataactga tcataatcag ccataccaca tt
//
