LOCUS       sequence-89045-e        1409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1409)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Rab11a-7
FEATURES             Location/Qualifiers
     source          1..1409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..689
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacggtcaga tccgctagcg ctaccggtcg ccaccatggt gagtgtgatt aaaccagaga
       61 tgaagatgag gtactacatg gacggctccg tcaatgggca tgagttcaca attgaaggtg
      121 aaggcacagg cagaccttac gagggacatc aagagatgac actacgcgtc acaatggccg
      181 agggcgggcc aatgcctttc gcgtttgact tagtgtcaca cgtgttctgt tacggccaca
      241 gagtatttac taaatatcca gaagagatac cagactattt caaacaagca tttcctgaag
      301 gcctgtcatg ggaaaggtcg ttggagttcg aagatggtgg gtccgcttca gtcagtgcgc
      361 atataagcct tagaggaaac accttctacc acaaatccaa atttactggg gttaactttc
      421 ctgccgatgg tcctatcatg caaaaccaaa gtgttgattg ggagccatca accgagaaaa
      481 ttactgccag cgacggagtt ctgaagggtg atgttacgat gtacctaaaa cttgaaggag
      541 gcggcaatca caaatgccaa atgaagacta cttacaaggc ggcaaaagag attcttgaaa
      601 tgccaggaga ccattacatc ggccatcgcc tcgtcaggaa aaccgaaggc aacattactg
      661 agcaggtaga agatgcagta gctcatagct ccggactcag atctcgagcc atgggcaccc
      721 gcgacgacga gtacgactac ctctttaaag ttgtccttat tggagattct ggtgttggaa
      781 agagtaatct cctgtctcga tttactcgaa atgagtttaa tctggaaagc aagagcacca
      841 ttggagtaga gtttgcaaca agaagcatcc aggttgatgg aaaaacaata aaggcacaga
      901 tatgggacac agcagggcaa gagcgatatc gagctataac atcagcatat tatcgtggag
      961 ctgtaggtgc cttattggtt tatgacattg ctaaacatct cacatatgaa aatgtagagc
     1021 gatggctgaa agaactgaga gatcatgctg atagtaacat tgttatcatg cttgtgggca
     1081 ataagagtga tctacgtcat ctcagggcag ttcctacaga tgaagcaaga gcttttgcag
     1141 aaaagaatgg tttgtcattc attgaaactt cggccctaga ctctacaaat gtagaagctg
     1201 cttttcagac aattttaaca gagatttacc gcattgtttc tcagaagcaa atgtcagaca
     1261 gacgcgaaaa tgacatgtct ccaagcaaca atgtggttcc tattcatgtt ccaccaacca
     1321 ctgaaaacaa gccaaaggtg cagtgctgtc agaacatcta aggatccacc ggatctagat
     1381 aactgatcat aatcagccat accacattt
//
