LOCUS       sequence-89048-e        1343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1343)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Talin-ABM-10
FEATURES             Location/Qualifiers
     source          1..1343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..682
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagtgtg attaaaccag agatgaagat
       61 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac
      121 aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg ccgagggcgg
      181 gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagagtatt
      241 tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc
      301 atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag
      361 ccttagagga aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga
      421 tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc
      481 cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa
      541 tcacaaatgc caaatgaaga ctacttacaa ggcggcaaaa gagattcttg aaatgccagg
      601 agaccattac atcggccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagcaggt
      661 agaagatgca gtagctcata gctccggact cagatctcga gctcaagctt cgatcctaga
      721 agctgccaag tccatcgctg cagccaccag tgcactggta aaggctgcgt cagcagccca
      781 gagggagctg gtggctcaag gaaaggtggg cgccattcca gccaatgcac tggatgatgg
      841 gcagtggtcg cagggcctca tttccgctgc ccgtatggtg gctgcggcca ccaacaatct
      901 gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc caggagaaac tcatttcctc
      961 agccaagcaa gtagctgcct ccacagctca gctcctggta gcttgcaagg tcaaagcaga
     1021 tcaggactct gaggcaatga aacggctcca ggctgctggc aatgcagtga agagggcctc
     1081 agataacctg gtaaaggcgg cccagaaagc tgcagccttc gaagaccagg agaatgagac
     1141 ggtggtggtg aaggagaaaa tggttggggg cattgcccag attatcgcag cacaggaaga
     1201 gatgcttcgg aaggaacgag agctggaaga ggctcggaaa aagctcgccc agatccggca
     1261 gcagcagtac aagttcttgc cttcagagct tcgagacgag cactaaggat ccaccggatc
     1321 tagataactg atcataatca gcc
//
