LOCUS       sequence-89049-e        7100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7100)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..689
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gaacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagtgtgatt aaaccagaga
       61 tgaagatgag gtactacatg gacggctccg tcaatgggca tgagttcaca attgaaggtg
      121 aaggcacagg cagaccttac gagggacatc aagagatgac actacgcgtc acaatggccg
      181 agggcgggcc aatgcctttc gcgtttgact tagtgtcaca cgtgttctgt tacggccaca
      241 gagtatttac taaatatcca gaagagatac cagactattt caaacaagca tttcctgaag
      301 gcctgtcatg ggaaaggtcg ttggagttcg aagatggtgg gtccgcttca gtcagtgcgc
      361 atataagcct tagaggaaac accttctacc acaaatccaa atttactggg gttaactttc
      421 ctgccgatgg tcctatcatg caaaaccaaa gtgttgattg ggagccatca accgagaaaa
      481 ttactgccag cgacggagtt ctgaagggtg atgttacgat gtacctaaaa cttgaaggag
      541 gcggcaatca caaatgccaa atgaagacta cttacaaggc ggcaaaagag attcttgaaa
      601 tgccaggaga ccattacatc ggccatcgcc tcgtcaggaa aaccgaaggc aacattactg
      661 agcaggtaga agatgcagta gctcatagct ccggactcag atctcgagct caagctagct
      721 tcgaattcgc caccatgcag cagtacaacc gagtggggaa agtggagcac ggctctgtgg
      781 ctctgccagc catcatgcgc tctggagcct ctggtcctga gaatttccag gtggggagca
      841 tgccacctgc ccagcagcag atcaccagtg gccagatgca ccgaggacac atgccacctc
      901 tgacttcagc ccagcaggcg ctcactggaa ccattaactc cagcatgcag gctgtgcagg
      961 ctgcccaggc cactctggat gactttgaga ctctaccccc tcttggccag gatgctgcct
     1021 ccaaagcttg gcgtaagaac aagatggatg aatcaaagca tgagatccac tcccaggtag
     1081 atgccatcac agctggcact gcctctgtgg taaacctgac agcaggagat cctgcagaga
     1141 cagactatac agcagtgggc tgtgcagtca ccaccatctc ttccaacctg acggagatgt
     1201 cccgtggggt gaagctattg gctgccctgc tggaggatga aggcggcaat ggccggcccc
     1261 ttctgcaagc agcaaagggc cttgcagggg ctgtgtcgga actgcttcgc agtgctcagc
     1321 ccgccagcgc tgagcctcgt cagaacctgc tgcaggcagc cgggaacgtg ggccaggcca
     1381 gtggggagct gttgcagcaa attggggaaa gtgacactga cccccacttc caggatgttc
     1441 taatgcagct agcaaacgca gtggcaagtg ctgcagccgc cctggtcctc aaggccaaga
     1501 gtgtggccca gcgcacagag gattccgggc ttcagaccca agtgattgct gcagctacgc
     1561 aatgtgctct gtccacttcc cagctggtgg cctgtaccaa ggtggtagca cctacaatca
     1621 gctcacctgt gtgccaagaa cagctagtcg aggctgggcg actggtggcc aaagctgtgg
     1681 agggctgtgt gtctgcctcg caagcagcta cagaagatgg acagctgctt cgaggggtag
     1741 gagcagcagc cacggctgtc acccaggccc tcaatgagct gctgcagcac gtgaaggccc
     1801 acgccaccgg agctgggcct gccggtcggt atgaccaagc cactgatacc atcctcaccg
     1861 tcactgaaaa catcttcagc tccatgggtg atgctgggga gatggtgcgc caggcccgca
     1921 tcctggccca agccacctca gacctggtca atgctatcaa ggccgatgct gagggggaga
     1981 gtgatctgga gaactctaga aagctcctga gtgctgccaa gatcctcgct gatgccaccg
     2041 ccaagatggt ggaggcggcc aagggagcag ccgcccaccc tgacagtgag gaacagcagc
     2101 agcgactgcg tgaagcagct gaggggcttc gcatggccac caatgcagct gcgcagaacg
     2161 ccatcaagaa gaagttggtg cagcgcctgg agcatgcagc caagcaagct gcagcctctg
     2221 caacacagac cattgctgca gcccaacatg cagcctctgc ccccaaggcc tctgcgggcc
     2281 cccagcccct actggtgcag agctgtaagg ccgtggcaga gcagattccg ttgctggtgc
     2341 agggtgtccg aggaagtcaa gctcagcctg acagccctag tgctcagctc gccctcattg
     2401 ctgccagcca gagcttcctg cagccaggtg ggaagatggt ggcagcggca aaagcctcag
     2461 tgccaacaat tcaggaccag gcttcagcga tgcagctgag tcagtgcgca aagaacctag
     2521 gcactgccct ggccgagctg cgcactgctg ctcagaaggc tcaggaggca tgtggacctt
     2581 tggagatgga ttctgcactg agtgttgtac aaaatctaga gaaagaccta caggaaataa
     2641 aagcagcagc tcgagatggc aagcttaagc ccttacctgg ggaaacaatg gagaagtgta
     2701 cccaagatct tggcaacagc accaaagcgg tgagctctgc catcgccaag ctgctggggg
     2761 agattgccca gggcaatgag aactatgcag gtattgcagc tcgggatgta gctggtggac
     2821 taaggtcact agcccaggct gcgcggggtg tggctgcgct gacatcagat cctgcagtgc
     2881 aggccattgt gcttgacaca gccagcgacg ttctggacaa ggccagcagc ctcattgaag
     2941 aggcaaaaaa agcatctgga cacccagggg acccagaaag ccagcaaagg cttgctcagg
     3001 tagctaaagc agtgacccaa gccctgaacc gctgtgtcag ctgcctgcct ggccaaagag
     3061 acgtggataa tgccctacga gccgttggag atgccagcaa gcgactcctg agtgacttgc
     3121 ttcctccaag cacggggaca tttcaagaag cacagagccg attgaatgaa gctgcagctg
     3181 ggttaaatca ggccgccaca gaactggtgc aagcctcccg aggaactcct caggacctgg
     3241 ctcgggcctc cggccgattt ggacaggact tcagcacctt cctggaagct ggtgtggaga
     3301 tggccggaca ggccccgagc caggaggacc gagcccaggt ggtgtccaac ctgaagggca
     3361 tatccatgtc ttcaagcaaa cttctccttg ccgctaaggc cttgtccaca gaccctgctt
     3421 ctcccaacct caagagtcag ctggctgcgg ctgcccgggc agtgacggac agcatcaacc
     3481 agctcatcac catgtgcacc cagcaggcac ctggccagaa ggagtgtgac aatgcacttc
     3541 ggcagctaga gacagtccga gaactcctgg agaatccagt ccagcccatc aacgacatgt
     3601 cctacttcgg ttgcttggac agtgtcatgg agaactccaa ggtcctaggt gaggccatga
     3661 ctggcatctc ccaaaatgcc aagaatggaa atctgccgga gtttggagat gccattgcca
     3721 cagcctccaa ggctctctgt ggcttcacgg aggcagccgc acaggcagca tatctagttg
     3781 gtgtctctga ccccaacagc caagctggac agcaaggact ggtggaaccc acacagtttg
     3841 cccgtgcaaa ccaggcaatt cagatggcct gtcagagtct gggggagcct ggctgtaccc
     3901 aggcccaggt gttatctgca gccactattg tagccaaaca cacatctgca ttgtgtaaca
     3961 gctgtcgcct ggcttccgct aggactgcca atcccactgc caagcgccag tttgtacagt
     4021 cagccaagga ggtggccaac agtacagcca atcttgtcaa gaccatcaag gcactagatg
     4081 gggacttcac agaagagaac cgtgcccagt gccgagcagc cacagcccct ctgctggaag
     4141 ctgtggataa cctgagtgcc tttgcctcca accctgagtt ctccagcgtc cctgcccaga
     4201 tcagccctga gggcagagca gccatggagc ccattgtaat ctctgctaag acaatgttgg
     4261 agagtgctgg agggctcatc cagacagctc gggcgttagc agtcaatccc cgagaccccc
     4321 cacgttggtc tgtgctagct ggccactctc ggactgtctc agattccatc aagaaactta
     4381 ttacaagcat gagggacaaa gccccagggc agctggagtg tgagacagcc attgcggctc
     4441 tgaacagctg cttgcgggac ctagatcagg cttcgcttgc cgctgtcagc cagcagcttg
     4501 ctccccgtga aggaatctct caagaggctt tgcacaccca gatgctcact gcagtgcaag
     4561 aaatttctca tctcattgag ccgctggcca gcgctgctcg ggctgaagcc tcccagctgg
     4621 gacacaaggt gtcccaaatg gcccagtact ttgaaccact caccctggct gcagtgggtg
     4681 ctgcgtctaa gaccctgagc cacccgcagc agatggcact tctggaccag actaaaacgt
     4741 tggcagagtc tgccttgcag ttgctatata ctgccaagga ggctggtggt aaccccaagc
     4801 aagcagcaca cacccaggaa gccctggagg aggctgtgca gatgatgaca gaggccgtag
     4861 aagacctgac gacgaccctc aacgaagcag ccagtgctgc aggagtcgtt ggcggcatgg
     4921 tggactctat cactcaggcc atcaaccagc tagatgaagg acctatgggt gacccagaag
     4981 gctcattcgt agattaccag acaaccatgg tgaggacagc caaggccatt gctgtcactg
     5041 ttcaggagat ggtaaccaag tcaaacacca gccctgaaga gctaggccct cttgccaacc
     5101 agctgaccag tgactatggc cgactggcct cacaagccaa gcctgcagct gtggctgctg
     5161 aaaatgaaga gataggcgct cacatcaaac accgagtaca ggagctgggc catggctgct
     5221 ctgctctggt caccaaggca ggtgccttgc agtgtagccc cagtgatgtc tacaccaaga
     5281 aggagctcat agagtgtgcc cgcagagtgt cagagaaggt ctcccatgtt ctggctgcac
     5341 tccaggctgg gaatcgtggt acccaggcct gcattacagc agccagtgct gtgtctggta
     5401 tcattgctga cctcgacacc accatcatgt tcgctactgc tggcacactt aaccgtgagg
     5461 gtgctgaaac ttttgctgac caccgggagg gtatcttaaa gacagcaaag gttcttgtgg
     5521 aggacaccaa ggtcctagtg cagaatgcag ctgggagcca ggagaagttg gcacaagccg
     5581 cccagtcctc cgtggccacc attacccgcc tcgctgatgt ggtcaagctc ggtgcagcca
     5641 gcctaggagc cgaagaccct gaaactcagg tggtgctgat caatgcagta aaggacgtag
     5701 ccaaggccct gggtgacctc atcagcgcta cgaaggctgc agcgggcaaa gttggggatg
     5761 accctgcagt gtggcagctc aagaactctg ccaaggtgat ggtgaccaat gtgacatcat
     5821 tgctcaagac agtgaaggct gtggaagatg aggccaccaa aggcacacgg gccctagaga
     5881 agacctctac ccctgaagac ttcatccgaa tgaccaaggg tattactatg gcaacagcca
     5941 aagccgttgc tgctggcaat tcctgtcgac aggaagatgt cattgccaca gccaatctga
     6001 gccgacgggc tattgcggac atgcttcggg cttgcaagga agcagctttc cacccagaag
     6061 tggcgcctga tgtacggctc cgagccctgc actatggccg ggagtgtgcc aatggttacc
     6121 tggaactgct ggaccacgtg ctgctgaccc ttcagaagcc aaacccagac ctgaagcagc
     6181 agctaactgg acactcaaag cgggttgctg gctctgtgac tgagctcatc caggctgctg
     6241 aagccatgaa aggaacagag tgggtggacc cagaggaccc tactgtcatt gctgagaatg
     6301 agctcttggg agccgcagcc gccatcgagg ctgcagccaa gaagctggag cagctgaagc
     6361 ccagggccaa acccaaggag gcggatgagt ccttgaactt tgaggaacaa atcctagaag
     6421 ctgccaagtc catcgctgca gccaccagtg cactggtaaa ggctgcgtca gcagcccaga
     6481 gggagctggt ggctcaagga aaggtgggcg ccattccagc caatgcactg gatgatgggc
     6541 agtggtcgca gggcctcatt tccgctgccc gtatggtggc tgcggccacc aacaatctgt
     6601 gtgaagcagc taacgcagct gtccagggcc atgctagcca ggagaaactc atttcctcag
     6661 ccaagcaagt agctgcctcc acagctcagc tcctggtagc ttgcaaggtc aaagcagatc
     6721 aggactctga ggcaatgaaa cggctccagg ctgctggcaa tgcagtgaag agggcctcag
     6781 ataacctggt aaaggcggcc cagaaagctg cagccttcga agaccaggag aatgagacgg
     6841 tggtggtgaa ggagaaaatg gttgggggca ttgcccagat tatcgcagca caggaagaga
     6901 tgcttcggaa ggaacgagag ctggaagagg ctcggaaaaa gctcgcccag atccggcagc
     6961 agcagtacaa gttcttgcct tcagagcttc gagacgagca ctaaaaaagc cctgtgtatt
     7021 taatgcagac ccagcccaga gactgtgcct gccactacca aagccttctg ggctgctcag
     7081 gacccaagct gcccaacccc
//
